摘要
样品用乙酸乙酯提取、酸性氧化铝去脂、碳酸钾溶液反萃取、乙酸酐衍生后，正己烷萃取衍生产物，气相色谱-ECD检测器测定，外标法定量。结果表明：3种化合物在5～100μg/L范围内线性关系良好，相关系数R2＞0.999，检测限为5μg/kg，对硝基酚钠的回收率为86.4％～89.6％，邻硝基酚钠的回收率为82.8％～84.3％，2-甲氧基-5-硝基苯酚钠的回收率为89.9％～92.2％，相对标准偏差均＜15％。该方法适用于鱼和虾可食部分中复硝酚钠的常规残留检测。
After the samples were extracted by ethyl acetate,the supernatant were cleansed by acidic a-lumina and extracted by potassium carbonate.The extracts were then reacted with acetic anhydride.The products were detected by gas chromatography with ECD detector via an external standard method follow-ing being extracted by n-hexane.The linearity of three compounds were held in 5 ~1 00 μg/L,with cor-relation coefficient R2 〉0.999 .The detection limits for the GC method （S/N=3 ）of the nitrophenolate were 5 μg/kg.The tests for recovery were carried by addition of standard at concentration of 1 0 ~1 00μg/kg.The result indicated that the residue content of p-nitrophenol sodium,o-nitrophenol sodium and 2-methoxy-5-nitropenolate sodium were 86.4% ~89.6%,82.8% ~84.3% and 89.9% ~92.2%, respectively,while the relative standard deviations were less than 1 5%.This method can be used to de-termine the residue content of nitrophenolate in edible portion of fish and shrimp.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第6期104-109,共6页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家"十二五"科技支撑计划资助项目(2012BAD29B06)
关键词
气相色谱法
鱼
虾
复硝酚钠
残留
Gas chromatography
fish
shrimp
sodium nitrophenolate
residue
