摘要
以广西火桐[Erythropsis kwangsiensis(Hsue)Hsue]叶片为试料,比较了改良CTAB法、试剂盒法和改良SDS法提取基因组DNA的提取效果及对干燥叶和鲜叶提取的基因组DNA进行了比较研究。结果表明,改良CTAB法和试剂盒法提取的基因组DNA质量较好、杂质少,适合广西火桐基因组DNA的提取。改良SDS法提取的基因组DNA无完整条带,质量较差,不适合广西火桐基因组DNA提取。硅胶干燥90 d的叶样和新鲜叶样所得的基因组DNA纯度相当,产率前者多于后者。
Using the leaves of Erythropsis kwangsiensis as material, the genomic total DNA was extracted. Three methods of genomic total DNA extraction including improved CTAB method, reagent box method and improved SDS method were compared.Results showed that the DNA extracted from improved CTAB method and reagent box method had high quality and less impurity, but quantity of the latter was less than that of the former. The reagent box method had the advantages of simple operation, short time and less harmful, but more expensive than that of the improved CTAB method. In addition, improved SDS method didn＇t have the complete bands, so it was not suitable for genomic DNA extraction of Erythropsis kwangsiensis.The genomic DNA extracted from the leaves preserved in silica gel for 90 days were not significantly different from that of fresh leaves, and the yield of the former was much more than that of the latter.
出处
《湖北农业科学》
北大核心
2013年第20期5060-5062,共3页
Hubei Agricultural Sciences
基金
广西植物研究所基本业务费支持项目(桂植业12009)
广西自然科学基金项目(2012GXNSFAA053069)
广西科技创新能力与条件建设项目(桂科能11217028)
科技部工作专项(2009FY120200)
