摘要
以疫霉属果胶裂解酶(pectin lyase,PL)基因家族的蛋白序列为材料,用邻接法和最小进化法构建系统发育树,根据所形成的分支确定PL基因家族的类群和分布规律。研究了疫霉属(Phytophthora)的果胶裂解酶基因家族的类群及在病原菌间的分布规律,以期为研究病原菌果胶裂解酶的专化性和病原菌的寄主专化性的关系奠定基础。结果表明:所构建的疫霉属PL基因家族的系统发育树共形成了11个分支,分别将其命名为PL1~PL11。其中,致病疫霉、橡树疫霉和大豆疫霉等3个病原疫霉属所共有的分支为5个,即PL1、PL6、PL7、PL10和PL11。橡树疫霉和大豆疫霉共有的分支为PL8。而上述3种疫霉均具其独有的1~2个分支,即PL4为橡树疫霉特有的分支,PL3和PL5为大豆疫霉的特有的分支,而PL2和PL9是致病疫霉特有的分支。利用疫霉果胶裂解酶基因家族的系统发育树的分支对其进行了分类和命名,并探究了疫霉属病原菌果胶裂解酶基因家族的分化与其寄主专化性的关系。
The protein sequences of pectin lyase gene family of Phytophthora were used to construct phylogenetic tree using neighbor-joining and minimum-evolution methods. According to the formed clades,classification and distribution of PL gene family were determined. The objective of this study was to investigate the classification of PL gene family of Phytophthora fungi and the gene distribution among different species of pathogens, which could laid the foundation of the relationship between the species specificity of pectin lyase genes and host specificity of pathogens. The results showed that the tree was divided into 11 clades,which were nominated as PLI^PLll, repectively. Of 11 clades, 5 ones （i. e. PL1, PL6,PL7 ,PLIO and PLll） were shared by three Phytophthora species i. e. P. infestans, P. sojae and P. ranzorum and PL8 was shared by P. sojae and P. ramorum. Nevertheless,three Phytophthora species had species-specific one or two clades. PL4 was specific to P. ramorum, PL3 and PL5 were specific to P. sojae and PL2 and PL9 were specific to P. infestans. The clades of phylogenetic tree were used to classify and nominate PL gene family of genus Phytophthora. The relationship of the division of PL gene family of Phytophthora pathogens and host specificity of pathogens was analyzed.
出处
《北方园艺》
CAS
北大核心
2013年第23期109-112,共4页
Northern Horticulture
基金
国家自然科学基金青年基金资助项目(31000833)
国家公益性行业(农业)科研专项资助项目(201303018)
