摘要
为明确玉米弯孢叶斑病菌的多聚半乳糖醛酸酶基因Clpg1在其致病过程中的作用,采用Double-joint PCR技术构建融合片段,利用PEG介导的原生质体转化技术获得18株抗潮霉素突变体,经检测得到1株Clpg1基因敲除突变体ΔClpg1。通过与野生型菌株比较,发现ΔClpg1产孢量和孢子萌发率下降、多聚半乳糖醛酸酶活性降低,致病力减弱。这些结果表明Clpg1基因影响多聚半乳糖醛酸酶活性,调控了玉米弯孢叶斑病菌的致病性。
In order to clarify the role of polygalacturonase Clpg1 gene in the pathogenicity of Curvularia lunata, the fusion fragment was constructed using double-joint PCR, and 18 hygromycin-resistant mutants were obtained by using PEG-mediated protoplast transformation technique. A knock-out mutant ΔClpg1 which contained no Clpg1 gene was found. Compared with the wild-type strain, the sporulation yield and germination rate of ΔClpg1 decreased;the polygalacturonase activity was reduced, and the pathogenicity was weakened. These results indicate that the Clpg1 gene affects the polygalacturonase activity and regulates the pathogenicity of Curvularia lunata.
作者
崔佳
赵丰舟
刘震
曲建楠
刘铜
左豫虎
CUI Jia;ZHAO Fengzhou;LIU Zhen;QU Jiannan;LIU Tong;ZUO Yuhu(College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Institute of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China)
出处
《植物保护》
CAS
CSCD
北大核心
2019年第6期163-169,184,共8页
Plant Protection
基金
海南大学高层次人才科研启动基金(KYQD(ZK)1851)
