摘要
目的：建立同时测定艾叶及其炮制品（醋艾叶、艾叶炭、醋艾炭）中棕矢车菊素和异泽兰黄素含量的方法，为控制艾叶及其炮制品质量提供新的评价指标。方法：采用反相高效液相色谱法。色谱柱为Agilent Eclipse XDB-C18（250mm×4．6mm，5gm），流动相为乙腈-0．2％磷酸溶液（37：63，V/V），流速为1．0ml／min，柱温为25℃，检测波长为330nm。结果：棕矢车菊素和异泽兰黄素的质量浓度分别在0．0140．20、0．01～0．16mg／ml范围内与各自峰面积积分值呈良好的线性关系（r分别为0．9995、0．9999）；精密度、稳定性、重复性试验的RSD〈3％；平均加样回收率分别为99．72％、101．19％，RSD分别为1．48％、0．90％（n均为6）。两种成分在艾叶、醋艾叶中含量较高，在艾叶炭和醋艾炭中含量极低或未检测到。结论：该方法简便、易行，测定结果准确、重复性好，可用于评价和控制艾叶和醋艾叶饮片的质量。
OBJECTIVE： To develop a method for simultaneous determination of eupatilin and jaceosidin in Artemisia argyi and its processed products（A, argyi acetum,A, argyi carbonisata,A, argyi carbonisata with vinegar）, and to provide new evaluation index for quality control of A. argyi and its processed products. METHODS： RP-HPLC method was adopted. The determination was performed on Agilent Eclipse XDB-C18 column（250 mm×4.6 mm, 5μm） with mobile phase consisted of acetonitrile-0.2% phosphoric acid（37 ： 63, V/V）at the flow rate of 1.0 ml/min. The column temperature was 25℃ and detection wavelength was set at 330 nm. RESULTS： The linear ranges were 0.01-0.20 mg/ml for eupatilin（r=0.999 5） and 0.01-0.16 mg/ml for jaceosidin（r=0.999 9）. RSDs of precision, reproducibility and stability tests were lower than 3%. The average recoveries were 99.72% （RSD=1.48 %, n=6） and 101.19% （RSD=0.90%, n=6）. The content of eupatilin and jaceosidin were higher in A. argyi and A. argyi acetum, while in A. argyi carbonisata and A. argyi carbonisata with vinegar, the two compound were not detected or very low. CONCLUSIONS ： The method is simple, convenient, accurate and reproducible, and can be used for quality control and evaluation of A. argyi and A. argyi acetum decoction piece.
出处
《中国药房》
CAS
CSCD
2013年第47期4464-4466,共3页
China Pharmacy
基金
国家自然科学基金资助项目(No.81173545)
国家中医药管理局中医药行业科研专项项目(No.200807039)
<山东省中药炮制规范>2012版标准研究课题
