摘要
目的对滇产阿坝当归干燥根部的化学成分及细胞毒活性进行研究,以期发现新的具有抗肿瘤活性的化学成分.方法用硅胶柱层析、Sephadex LH-20凝胶柱层析、Rp-18反相柱层析、半制备HPLC方法对阿坝当归根部的甲醇提取物进行分离、纯化,用MTT测定单体化合物对K562、SMMC-7721两株人肿瘤细胞增殖的抑制作用.结果从阿坝当归根部获得8个单体化合物,其中7个化合物为线型呋喃香豆素类型.结论滇阿坝当归含有丰富的香豆素成分,但经体外抗肿瘤活性的初步筛选,分离得到的7个线型呋喃香豆素化合物体外抑制肿瘤细胞增殖作用不明显.
Objective Obtaining chemical constituents of Angelica apaensis collected in Qiaojia County of Yunnan Province and detecting their anti-tumor activities in vitro. Methods The MeOH extract of A. apaensis was repeatedly subjected to column chromatography over silica gel, Sephadex LH-20 and/or RP-18 and semipreparative HPLC. Their anti-tumor activities in vitro were measured by MTT colorimetric assay. Results Eight compounds were isolated from the roots of this plant. Conclusion The major type of chemical constituents of A. apaensis in Yunnan is furocoumarin. These separated compounds showed weak anti-tumor activity in vitro.
出处
《昆明医科大学学报》
CAS
2013年第11期1-4,共4页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(21102060)
云南省应用基础研究基金资助项目(2011FB058)
