摘要
目的对滇产隆萼当归化学成分及细胞毒活性进行研究,以期发现新的具有抗肿瘤活性的微量成分.方法经硅胶柱层析、半制备HPLC方法获得7个单体化合物,用MTT测定7个单体化合物对2株人肿瘤细胞的增殖抑制作用.结果线型呋喃香豆素类单体化合物2和6能明显抑制2株人肿瘤细胞的增殖,其IC50值均小于2μg/mL,作用甚至强于顺铂.结论滇产隆萼当归中的2种线型呋喃香豆素类成分具有较好的体外抗肿瘤活性,结果表明,从云南伞形科药用植物中分离抗肿瘤活性成分有着较好的前景.
Objective To study the chemical constituents of A. oncosepala collected in Lijiang County of Yunnan Province and to evaluate their anti-tumor activities in vitro. Methods By repeated column chromatography and semipreparative HPLC, 7 compounds were separated. Their anti-tumor activities in vitro were measured by MTY eolorimetric assay. Results Compounds of linear furocoumarins 2 and 6 obviously inhibited proliferation of 2 human tumor cell lines, their ICS0 values were equal or lower than cisplatin. Conclusion 2 linear furocoumarins from Yunnan A. oncosepala exhibit excellent anti-tumor activity in vitro, indicating that constituents from Angelica genus nlants may be good resource for new anti-tumor compounds.
出处
《昆明医学院学报》
2012年第6期25-27,31,共4页
Journal of Kunming Medical College
基金
云南省高校药学重点实验室项目(2010ZL004)
云南省教育厅科学研究基金资助项目(2010Y191)
关键词
隆萼当归
线型呋喃香豆素
抗肿瘤活性
Angelica oncosepala
Linear furocoumarins
Anti-tumor activity
