摘要
以小鼠骨骼肌母细胞(C2C12)肌管为研究对象,测定不同时间电刺激对其AMPK、HK-Ⅱ、GS-1基因表达、GLUT4基因及蛋白表达等糖转运和代谢相关指标的变化,探讨不同收缩时间对骨骼肌细胞糖代谢的影响及机制.骨骼肌细胞的肌糖原消耗随刺激时间延长而增加,为了保证骨骼肌细胞糖消耗的需要,通过AMPK信号通路的调控,其膜对胞外糖转运的能力会随收缩时间延长而增强;骨骼肌细胞内糖原合成酶的合成则随肌糖原储量的持续下降而增加.
The aim of this study was to measure the changes in cellular glucose intake and carbohydrate metabolism in mouse C2C12 skeletal muscle cells, and to investigate the effect on skeletal muscle cells carbohydrate metabo- lism by electrical stimulation of different times as well as the possible mechanism. Glycogen consumption increased continuously with prolonged contraction of skeletal muscle cells. The capacity of glucose translocation across the cell membrane was enhanced with the increase of contraction time. The activity of glycogen synthase was increased due to declining muscle glycogen reserves after 60 and 120 min electrical stimulation. And all of these changes were pos- sibly regulated by the AMPK signaling pathway.
出处
《华南师范大学学报（自然科学版）》
CAS
北大核心
2013年第6期155-160,共6页
Journal of South China Normal University(Natural Science Edition)
基金
广东省体育局2012~2013奥运全运专项攻关项目(201220NS046)
关键词
骨骼肌细胞
电刺激
糖代谢
信号转导
skeletal muscle cells
electrical stimulation
carbohydrate metabolism
signaling pathway
