摘要
采用顶空固相微萃取结合GC–MS分析技术,对花蕾期、露白期、初放期和盛开期的枇杷花的挥发性物质进行富集、分离和鉴定,分别鉴定出挥发性化合物84、90、69和73种,各占总挥发性物质的93.84%、97.74%、92.56%和92.67%,都含有酮类、酸类、酯类、醛类、烃类、醇类和其他少量化合物。从花蕾到盛开过程中,酯类化合物相对含量呈不断上升趋势,而醛类和酸类物质相对含呈快速下降趋势,烃类和酮类先上升后下降。4–甲氧基苯甲酸甲酯、4–甲氧基苯甲酸乙酯、二–(2–苯乙基)–草酸酯、苯甲醛、甲氧基苯甲醛、反式二己烯醛、苯乙醇、十三烷、二十一烷等成分是枇杷花香的主要赋香物质,它们在枇杷花开放不同阶段的相对含量之和分别占总挥发性物质的55.03%、59%、72.68%和70.71%。枇杷花从露白期到初放期具有较丰富的香气组成,应以此时为较佳的利用时期。
Volatile compounds in four different stages of loquat flowers（the bud stage,the bud
swelling and come out white petals,the petals beginning spread and the petals opened）were extracted by
Headspace Solid Phase Microextraction（SPME）,seperated and analysed by GC–MS. As a result,84,
90,69 and 73 kinds of volatile compounds were identified from the four different stages of loquat flowers,
respectively,and accounted for 93.84%,97.74%,92.56% and 92.67% of the total volatile compounds.
Ketones,acids,esters,aldehydes,alkanes,alcohols and a few other compounds existed in the volatile
compounds. From the bud stages to the petals opened of loquat flowers,the content of the esters and acids
showed gradually increase,the content of aldehydes revealed a rapid decrease,and the content of akanes
and ketones exhibited an initial increase and a final decrease. In all volatile compounds of loquat flowers,
the main aromatic components were 4-methoxy-benzoic acid methyl ester,4-methoxy-benzoic acid ethyl
ester,oxalic acid di（2-phenylethyl）ester,benzaldehyde,4-methoxy-benzaldehyde,（E）-2-hexenal,
phenylethyl alcohol,tridecane,heneicosane,etc. These aromatic components accounted for 55.03%,59%,
72.68% and 70.71% of four different stages of loquat flowers,respectively. In conclusion,there were good aromatic components in loquat flowers from the bud swelling and come out white petals to the petals
beginning spread.
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第11期2245-2254,共10页
Acta Horticulturae Sinica
基金
国家林业局重点科技成果推广项目(2012-68)
财政部以大学为依托的农业科技推广模式建设项目(XTG2013-17)
关键词
枇杷
花
花期
香气物质
固相微萃取
气相色谱质谱联用
loquat；flowers；flower stages；aromatic components；solid phase microextraction（SPME）；gas chromatography-mass spectroscopy（GC＆ndash, MS）
