摘要
论述了用桔青霉PenicilliumcitrinumM71菌株 ,经液体培养制得的 5′ -磷酸二酯酶降解鲐鱼鱼精DNA成5′ -脱氧核苷酸的分离工艺。分离采用 2 0 1× 8阴离子交换树脂 ,具体条件为 :柱床高 1 0 5mm ,柱床直径 45mm ,样品浓度 2 1 3mg·mL-1,洗脱流速 0 .5mL·cm-2 ·min-1。分离结果表明 ,采用 0 .0 0 5MHCl+0 .0 4MNaCl作洗脱剂 ,流速为 0 .7mL·cm-2 ·min-1时 ,四种 5’ -脱氧核苷酸组分能完全被洗脱下来 ,且呈一个大峰 ,同其它成分分开 ,再先后采用 0 .0 0 1 8MHCl、0 .0 0 2 8MHCl、0 .0 36MNaCl(pH6 .0 )、0 .0 0 5MHCl+0 .0 2MNaCl作洗脱剂时 ,则能分别将dCMP、dAMP、TMP。
The process of digesting DNA-Na obtained from fish spermary of chub mackerel into 5′-Deoxymononucleotides by using 5′-phosphodiesterase from Penicillium citrinum, M71 was studied.The 201×8 anion exchange resin was used in the separation.The conditions for setting samples are height of column bed 105mm, diameter of column bed 45mm, sample concentration 213mg·mL -1 and eluting flow rate 0.5mL·cm -2 ·min -1 . The results of the separation indicated 5′-deoxymononucleotides components could be fully eluted as a whole peak by the eluting agent, 0.005 M HCl and 0.04M NaCl. And the use of a few modified eluting agents, i.e. 0.001 8M HCl,0.002 8M HCl, 0.036M NaCl(pH6.0) and 0.005M HCl+0.02M NaCl could bring about perfect and respective elution of dCMP, dAMP, TMP and dGMP.
出处
《水产学报》
CAS
CSCD
北大核心
2000年第3期275-279,共5页
Journal of Fisheries of China
基金
农业部渔业局资助项目 !(鲐鱼鱼精中 5'-脱氧核苷酸的分离工艺研究 )
