摘要
目的探讨XRCC1基因Arg399Gln位点多态性与宁夏回、汉族食管癌易感性的关系，以及基因与饮酒的交互作用对食管癌发病的影响。方法收集宁夏地区食管鳞癌患者88例为病例组，其中回族38例，汉族50例；正常对照组回族、汉族各50例。采用PCR—RFLP方法检测各组Arg399Gln位点基因型，比较病例组与对照组的等位基因及基因型频率，以比值比（OR）及95％其可信区间表示各种基因型及饮酒与食管癌风险的相关性。结果病例组和对照组XRCC1基因Arg399Gln等位基因频率A和G分别为72％、28％及75％、25％，3种基因型Arg／Arg、Arg/Gln、Gln／Gln分别为53％、38％、9％及60％、30％、10％，病例组与正常对照组比较差异无统计学意义（P〉0．05）。回、汉族比较，Arg399Gln位点等位基因频率及基因型频率在回、汉族食管癌病例分别为76．3％、23．7％和69％、3／％及60．5％、31．6％、7．9％和48％、42％、10％，两者间差异无统计学意义（P〉0．05）。病例组饮酒37例（42％），较对照组22例（22％）高，以不饮酒野生基因型Arg／Arg为对照，饮酒可增加野生基因型食管癌的发病风险P〈0．05，OR=4．158（95％CI1．647～10．499）；回、汉族病例进行比较，回族饮酒食管癌风险可增加2倍，OR=2．028（95％CI0．750-5．807）。结论XRCC1基因Arg399 Gln位点多态性与宁夏地区回、汉族食管癌的发病可能无关，但饮酒可能增加XRCC1基因Arg399Gln位点野生基因型的食管癌发病风险，回族风险更高。
Objective To investigate the polymorphism of Arg399Gln sites in X - ray repair cross complementing gene 1 （ XRCC1 ） and interaction with drinking to esophageal cancer （EC） susceptibility in Hui - Han people of Ningxia. Methods The blood specimen of 88 patients （38 cases of Hui people and 50 cases of Han people） with EC in Ningxia were collected; and the blood specimen of 50 cases of Hui people and 50 cases of Han people were in the control group. XRCC1 genotypes were detected by PCR - RFLP. Questionnaires were used to gathered individual information. Chi - square test was used to compare the distribution of the genotype between two groups. The adjusted odds ratio （OR） and 95% confidence interval （95% CI） was calculated the interaction risk factors between the gene and Alcohol Consumption. Results The frequencies of the XRCC1 codon 399 A and G allele among EC patients and healthy controls were 72%, 28% and 750/0 , 25% , respectively. The distribution of the Arg/Arg, Gln/Arg and G1n/G1n genotypes between EC patients were 53% , 38% and 9% in experiment group and 60% , 30% andl0% in controls, respectively. There was no significant difference in the XRCC1 Arg399 Gin distribution and genotypes between EC patients and controls （P 〉 0.05 ）. The allele distribution of the Arg/Arg, Gln/Arg and Gln/Gln between EC patients of Hui people and Han people were 76.3% , 23.7% and 69% , 31%, genotypes were 60.5%, 31.6% , 7.9% and 48% , 42%, 10%. There was no significant difference in the XRCC1 allele distribution and genotypes between EC patients of Hui and Han （ P 〉 0.05 ）. Combination Analysis the interaction risk factors of Alcohol Consumption and polymorphisms of genes showed that Alcohol Consumption increased the risk of esophageal cancer on the patients of XRCC1 399 codon Arg/Arg genotypes. The adjusted odds ratio （OR） and 95%confidence interval （95%CI） was 4. 158 （95% CI 1. 647 - 10. 499, P 〈 0.05 ）. Conclusion The XRCC1 eodon 399 SNPs might not be related to the risk of EC deve
出处
《宁夏医学杂志》
CAS
2013年第10期889-891,I0003,共4页
Ningxia Medical Journal
基金
宁夏自然科学基金资助项目(NZ10182)
关键词
食管癌
基因多态性
X射线
交叉互补修复基因1
交互作用
Esophageal cancer
Gene polymorphisms
X - ray
Repair cross complementing gene 1
Interaction
