摘要
采用大孔吸附树脂HPD826、ODS柱和Sephadex LH-20分离纯化得到高纯度的茶叶籽黄酮单体化合物Ⅰ,通过紫外(UV)、红外(IR)、质谱(MS)、核磁共振(NMR)等方法鉴定其分子结构为柚皮素-7-O-(β-D-吡喃木糖基(1→6))(β-D-葡萄糖基(1→3)-α-L-鼠李糖基(1→2))-β-D-葡萄糖苷。对化合物Ⅰ及其同系物柚皮素和柚皮苷的抗氧化活性进行对比研究。结果表明:化合物Ⅰ在体外有一定的抗氧化能力,3种物质的抗氧化活性关系为柚皮素>柚皮苷>化合物Ⅰ,即黄酮苷元的抗氧化活性大于黄酮糖苷,黄酮二糖苷的抗氧化活性大于黄酮四糖苷。
Using HPD826 resin、ODS and Sephadex LH-20 column chromatography, compound I, a flavanone glycoside, was obtained. The structure of compound I was identified determined by means of UV、IR、NMR and LC-MS as naringenin-7-O-[β-D-xylopyranosyl（1→6）][β-D-glucopyranosyl（1→3）-α-L-rhamnopyranosyl（1→2）]-β-D-glucopyranoside. The antioxidant activity of compound I in vitro was measured, compared with the standard of naringenin and naringin. Results show that the antioxidant activity in vitro of three materials in the order： naringenin 〉 naringin 〉 compound I, that is, the antioxidant activity of aglycon flavanone is greater than the flavanone glycosides, the antioxidant activity of diglycoside flavanone is greater than flavanone with four glycosyl moieties. This evidence suggested that the glycosyl moieties in flavanones reduced antioxidant activity.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第21期115-120,共6页
Food Science
关键词
茶叶籽
黄酮
鉴定
抗氧化
tea camellia seed
flavonoids
identification
antioxidant activity
