摘要
目的探究羊蹄根对体外肝星状细胞(HSCs)增殖的影响。方法将液氮下保存的肝星状细胞株于37℃、5%CO2孵育箱中复苏传代培养,加入转化生长因子(TGF-β)刺激HSCs 12h后,同步分为对照组和羊蹄根不同剂量(1∶1 200、1∶1 800和1∶2 400)组,2h后采用倒置荧光显微镜观察细胞形态以及MTT比色法检测细胞增殖情况。结果镜下可见羊蹄根组比对照组凋亡细胞显著增多,细胞密度明显减少,细胞由典型的星形变成了椭圆形或梭形,细胞体积减小,细胞结构完整,细胞之间伪足连接少见、融合基本消失;各剂量组对细胞增殖均有显著抑制作用(P<0.05),抑制率分别为69.36%、32.01%、29.14%,且高剂量组与中、低剂量组相比细胞抑制作用比较,差异有统计学意义(P<0.01)。结论羊蹄根对HSCs的增殖有显著抑制作用,能有效缓解HSCs的纤维化程度。
Objective To explore the root of Rumex japonicus on hepatic stellate cells （HSCs）proliferation in vitro. Methods The hepatic stellate cells under liquid nitrogen preservation were recovered at 37℃ and 5% CO2, and subcultured. The transforming growth factor （TGF-beta） was used to stimulate HSCs 12 h after HSCs cultures, Synchronized HSCs were divided into control group,the Rumex japonicus group at different doses （1 ： 1 200,1 ： 1 800,1 ： 2 400）, two hours after the treatment, the inverted fluorescence microscope was used to detect cell morphology and MTT colorimetry was used to detect ceil proliferation. Results Microscopically the apoptotic cells in Rumex japonicus group were increased compared to control group, cell density decreased significantly. The cell morphology was altered cell volume decreased, and cellular pseudopod connection disappeared. Rumex japonicas at different doses were effective in inhibition of cell proliferation （P〈0. 05）, the inhibition rates were 69. 36 %, 32. 01%,29.14%, respectively,and the inhibition rate at high dose group was highest among middle and low doses（P〈0.01）. Conclusion Rumex japonicus was effective in inhibition of HSCs proliferation and alleviates the degree of fibrosis HSCs.
出处
《成都医学院学报》
CAS
2013年第5期551-553,共3页
Journal of Chengdu Medical College
基金
吉林医药学院大学生科研基金项目(吉医学科字[2010]第8号)
