摘要
通过加入乙酸钠优化DNA提取方法,针对泡桐ITS的高GC含量模板,在反应体系中加入DMSO优化PCR反应体系,有效扩增了泡桐ITS序列。采用PCR直接测序和pGM-T载体克隆测序的方法相互验证序列,提高所得ITS序列的准确性,为采用泡桐ITS序列对泡桐的系统发育及遗传多样性等研究提供新的方法。
Through optimizing DNA extraction method by adding sodium acetate, taking into account the template with high GC content in the ITS of Paulownia, the DMSO and optimization of PCR reaction system were joined in the reaction system, thereby amplifiying the ITS sequence of Paulownia. The mutual verification between PCR direct sequencing and pGM-T vector clone sequencing raised the accuracy of the obtained ITS sequences. The results provide a new method for using ITS Paulownia sequences to research phylogeny and genetic diversity of Paulownia provide.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2013年第10期30-33,共4页
Journal of Central South University of Forestry & Technology
基金
林业公益性行业科研专项"泡桐基因库与育种群体建立技术研究"(200804021)
关键词
泡桐
ITS序列
特征分析
Paulownia
ITS sequence
characteristic analysis
