摘要
为了探究杜仲多居群的遗传多样性,通过开展正交试验,建立了适合杜仲的ISSR-PCR反应体系,筛选出了14条多态性较高的有效引物,分析了杜仲的遗传多样性;共扩增出108个多态性位点,多态性位点的百分率为100%,有效等位基因数ne为1.505,Nei’s期望杂合度He为0.308,Shannon多态性的信息指数I为0.472。文中初步揭示出杜仲具有较高的遗传多样性,试验结果表明了ISSRs标记技术适用于杜仲的遗传多样性研究。
In order for the further study on population genetic diversity in Eucomia ulmoides, inter-simple sequence repeat-polymerase chain reaction （ISSR-PCR） system was established through orthogonal test for E. ulmoides. Fourteen ISSR polymorphic primers were selected, and genetic diversity was analyzed. A total of 108 polymorphic loci were amplified, showing percentage ofpolymorphic loci of 100%, effective number of alleles per locus （ne） of 1.505, expected heterozygosity （He） of 0.308, and Shannon＇s information index （I） of 0.472. The results indicate that E. ulmoides has high level of genetic diversity, and ISSRs are suitable markers for genetic diversity analysis in E. ulmoides.
出处
《经济林研究》
北大核心
2013年第1期30-34,共5页
Non-wood Forest Research
基金
安徽高校省级自然科学重点项目(KJ2011A115)
