摘要
目的探讨北柴胡正丁醇萃取部位的保肝作用及与其HPLC指纹图谱间的谱效关系,以阐明其药效物质基础。方法采用HPLC-ELSD法获取北柴胡正丁醇萃取部位的指纹图谱;检测CCl4所致急性肝损伤小鼠血清中丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)水平、肝组织中丙二醛(MDA)的量和超氧化物歧化酶(SOD)的活性;利用聚类分析与典型相关分析法对色谱-药效数据进行相关分析。结果从HPLC指纹图谱中共提取出12个能够标示药材特征的皂苷类成分的共有峰。不同产地北柴胡药材的保肝作用存在显著差异(P<0.05)。聚类分析和典型相关分析明确了给予北柴胡正丁醇萃取部位的小鼠血清中ALT水平和肝组织中SOD活性与皂苷类化合物柴胡皂苷a和柴胡皂苷d之间存在线性关系。结论北柴胡保肝作用的部分药效物质基础为柴胡皂苷a和柴胡皂苷d。
Objective To analyze the spectrum-effect relationship between the hepatoprotective effect and HPLC fingerprints of the n-butanol fraction extracted from Bupleurum chinense and to elucidate its effective substance. Methods HPLC-ELSD was used to obtain the fingerprint peaks of n-butanol fraction of B. chinense; the levels of alanine transaminase （ALT） and aspartate aminotransferase （AST） in serum, malondialdehyde （MDA） content, and superoxide dismutase （SOD） activity in liver tissue of mice with acute hepatic injury induced by CC14 were detected. Cluster analysis and typical correlation analysis were used to analyze the data of spectrum and efficacy. Results Twelve common peaks which could show the features ofB. chinense were isolated and there was statistical difference （P 〈 0.05） among the hepatoprotective effects ofB. chinense from different origins. Saikoside a and saikoside d were proved to have linear relationship with the serum ALT level and liver tissue SOD activity in mice with acute liver injury in the treatment group by cluster analysis and typical correlation analysis. Conclusion Saikoside a and saikoside d are the effective substances ofB. chinense with hepatoprotective effect.
出处
《中草药》
CAS
CSCD
北大核心
2013年第19期2696-2702,共7页
Chinese Traditional and Herbal Drugs
基金
国家"十一五"科技支撑计划资助项目(2006BAIO06A10)
河南省科技成果转化项目(092201310001)
关键词
北柴胡
保肝作用
HPLC指纹图谱
谱效关系
药效物质基础
Bupleurum chinense DC.
hepatoprotective effect
HPLC fingerprint
spectrum-effect relationship
effective substance
