摘要
目的对细粒棘球绦虫5种天然抗原制剂的诊断效能进行评价。方法从绵羊肝棘球蚴囊中收集囊液和原头节,制备细粒棘球蚴原头节虫体可溶性抗原(EgPS)、囊液部分纯化抗原(EgBu)、囊液粗制抗原(EgHF)和天然抗原B(EgAgB)。从实验感染的家犬肠道中收集细粒棘球绦虫成虫,制备成虫虫体可溶性抗原(EgAS)。对369例细粒棘球蚴病患者,99例其他寄生虫病患者和366名健康体检者共834份血清样品,用间接ELISA技术检测抗体。检测结果经统计学处理后进行综合性分析和评价。结果5种天然抗原中,EgPS抗原的ROC曲线下面积、敏感性、阳性预测值、阳性似然比、诊断效率和诊断优势比最高,分别为0.958±0.067、88.7%、0.96、16.61、91.7%和138.475。特异性以EgBu抗原最高(96.1%)、余下依次为EgAgB(95.7%)、EgPS(94.7%)、EgAS(89.0%)和EgHF(80.4%)。约登指数的排序则为EgPS>EgBu>EgHF>EgAS>EgAgB。抗体反应强度以EgBu-ELISA最高(4.055±1.365),EgAS-ELISA最低(2.947±1.276),但两者差异无统计学意义(P>0.05)。ELISA检测结果显示,5种天然抗原与多房棘球蚴病患者血清有高度交叉反应(9/14~12/14),与囊尾蚴病患者血清的交叉反应阳性率也达8/20~16/20。结论不同抗原ELISA检测结果之间存在着明显差异。以EgPS抗原的诊断效能最佳,其次为EgBu和EgAgB抗原。
Objective To evaluate the diagnostic potential of 5 native antigens obtained from Echinococcus granulosus. Methods The cyst fluid and protoscoleces were collected from infected livers of slaughtered sheep near Urumqi. The protoscolex somatic soluble antigen （EgPS）, crude bydatid cyst fluid antigen （EgHF）, partially purified hydatid fluid antigen （Burstein＇s antigen, EgBu） and the native antigen B （EgAgB） were prepared. Echinococcus granulosus adult worms were collected from experimentally infected dogs, and then used to prepare adult somatic soluble antigen （EgAS）. Serum samples from patients with cystic echinococcosis （369 cases）, alveolar echinococcosis （14 cases）, cysticercosis （20 cases）, schistosomiasis（50 cases）, paragonimiasis westermani （10 cases）, visceral leishmaniasis（5 cases）, and healthy individuals（366 cases） were examined for the antibodies against 5 native antigens with indirect ELISA. A ROC analysis was performed to determine a cut-off value for each native antigen. Results EgPS presented the highest AUCR^c （0.958-x0.067）, sensitivity （88.7%）, positive predictive value （0.96）, positive likelihood ratio （16.61）, diagnostic efficiency （91.7%）, and odds ratio （138.475）. The specificity of EgBu （96.1%） was the highest, followed by EgAgB （95.7%）, EgPS （94.7%）, EgAS （89.0%）, and EgHF （80.4%）. The Youden index was ranked in order of EgPS〉EgBu〉EgHF〉EgAS〉EgAgB. The antibody reactive intensity of EgBu-ELISA was highest （4.055±1.365） and the EgAS-ELISA was lowest（2.947±1.276）, and there was no significant difference between them （P〈0.05）. The five native antigens presented high cross-reactivity with sera from alveolar echinococcosis （9/14- 12/14） and from cysticercosis （8/20-16/20）. Conclusion There are significant differences among the five native antigen- ELISA. The EgPS antigen has the best diagnostic performance , which is followed by EgBu and EgAgB.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2013年第5期357-362,共6页
Chinese Journal of Parasitology and Parasitic Diseases
基金
新疆维吾尔自治区科技厅科技支疆项目(No.200891130)~~
关键词
细粒棘球绦虫
天然抗原
诊断效能
酶联免疫吸附试验
棘球蚴病
Echinococcus granulosus Native antigen Diagnostic performance Enzyme-linkedimmunosorbent assay Cystic echinococcosis
