摘要
用细粒棘球绦虫成虫虫体抗原及其免疫家兔血清IgG建立了用于检测家犬粪抗原诊断细粒棘球绦虫成虫感染的双抗体夹心ELISA方法。对用离子交换层析、SPA亲和层析、抗兔IgG抗体亲和层析和细粒棘球绦虫成虫虫体抗原亲和层析等四种方法纯化的抗体进行了比较,以抗原亲和层析法纯化的抗体免疫活性最高,用双抗体夹心ELISA检测抗原的终点在标本缓冲液中为2.5ng/ml,在健康家犬粪便悬液的上清中为5ng/ml。检测11只人工感染细粒棘球绦虫成虫的家犬粪便标本,粪抗原阳性率达100%。
The establishment of method of double antibody sandwich ELISA for diagnosis of E.granulosus infection in dogs by using the adult somatic antigen-antibody system was reported. The methods of ion exchange chromatography, SPA affinity chromatography, anti-rabbit IgG antibody affinity chromatography and antigen affinity chromatography were used in antibody purification. The best immunoactivities were obtained in the antibodies purified by antigen affinity chromatography. The endpoint of antigen detection by double antibody-sandwich ELISA were 2.5ng/ml in sampling buffer and 5ng/ml in supernatant of dog faeces suspanded in sampling buffer respectively. Application of this method in detection of coproantigen in 11 experimentally infected dogs proved by arecoline purgation demonstrated 100% positive.
出处
《地方病通报》
1998年第2期16-21,共6页
Endemic Diseases Bulletin
基金
卫生部科研基金
关键词
细粒棘球绦虫
成虫
感染
粪抗原
检测
诊断
Echinococcus granulosus
Adult
Infection
Dog
Coproantigen
Detection