摘要
目的 评价粪抗原检测法在包虫病流行病学监测中的实际应用价值。 方法 家犬在采集粪便后 ,进行槟榔碱导泻 ,应用粪抗原检测法检测粪样中的细粒棘球绦虫抗原 ,并与槟榔碱导泻法的驱虫结果比较 ,同时分析犬肠道中寄生的其它蠕虫对粪抗原检测结果的影响。 结果 在 2 94只槟榔碱导泻成功的家犬中 ,4 5只检出细粒棘球绦虫 ,粪抗原阳性犬共 4 6只。二者的符合率为 97.8%。在 2 4 9只未检出细粒棘球绦虫的家犬中 ,粪抗原阳性者 1只。 结论 粪抗原检测法诊断犬细粒棘球绦虫感染具有较高的敏感性和特异性。可作为包虫病常规监测方法推广应用。
Objective To assess the value in field application of coproantigen detection in dogs for epidemiological surveillance of echinococcosis. Methods Comparison of results of arecoline purgation and coproantigen detection in fical samples collected before arecoline treatment and analyzing the influence of various helminthes parasitizing in gut of dogs to results of coproantigen detection. Results The adult worm of Echinococcus granulosus have been detected in 45 of 249 dogs purged by arecoline treatment. Coproantigen positive were seen in 46 dogs. The correspondent rate between arecoline purgation and coproantigen detection was 97.8%. The coproantigen positive only one in 249 dogs of negative in arecoline purgation. Conclusion The coproantigen determination has higher sensitivity and specificity in diagnosis of E. granulosus infection in dogs compared with arecoline purgation. It could be used as a routine method in surveillance of cystic echinococcsis.
出处
《中国寄生虫病防治杂志》
CSCD
2003年第3期170-171,共2页
Chinese Journal of Parasitic Disease Control
基金
新疆维吾尔自治区科技攻关
重点科技项目 (No.2 0 0 0 0 1 0 30 0 2 )。
关键词
粪抗原
检测
诊断
犬
细粒棘球绦虫感染
包虫病
Echinococcus granulosus
surveillance
echinococcsis
coproantigen
dog
double antibody sandwich ELISA
