摘要
通过静态吸附-解吸实验筛选适合西番莲果皮花色苷纯化的大孔树脂,并进行静态、动态吸附-解吸工艺条件优化。结果表明:在供试树脂中,HP-20大孔树脂为西番莲果皮花色苷纯化最适用的吸附剂,其静态吸附最优条件为温度40℃、pH4.0、洗脱液为90%酸化乙醇(pH3.5);动态吸附-解吸最适工艺条件为:上样液流速4.5BV/h、上样液质量浓度18.176μg/mL、洗脱剂用量6BV、洗脱流速3BV/h。
Static adsorption and desorption experiments were conducted to screen the suitable macroporous resin for purifying pigments from Passiflora edulis rind. The conditions for static and dynamic adsorption and desorption were optimized. Results showed that macroporous resin HP-20 was the most favorable adsorbent for Passiflora edulis rind anthocyanins. The optimal static adsorption and desorption conditions were adsorption at 40 ℃ of samples at pH 4.0 and desorption with 90% acidified ethanol （pH 3.5）. The optimal dynamic adsorption and desorption conditions were loading of 18.176 μg/mL sample solution at a flow rate of 4.5 BV/h and gradient desorption with 6 BV of the desorption solvent at a flow rate of 3 BV/h.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第20期61-66,共6页
Food Science
基金
福建省科技计划项目(2009C0022)
关键词
西番莲果皮
花色苷
HP-20大孔树脂
吸附
Passiflora edulis rind
anthocyanins
macroporous resin HP-20
adsorption
