摘要
目的 :建立山楂叶总黄酮有效成分的含量测定方法 ,完善其质量标准。方法 :应用反相高效液相色谱法测定了山楂叶总黄酮中牡荆素的含量。 C1 8柱作为色谱柱 ,乙腈 -3 %醋酸水溶液 ( 11∶ 89)为流动相 ,检测波长 2 70 nm,按外标法定量。结果 :样品经提取分离水解 ,去掉大部分杂质 ,使达到基线分离。6次平均加样回收率为 99.0 9% ,RSD=3 .76% ;重现性试验的 RSD=3 .66% ( n=6)。结论 :为山楂叶总黄酮提供了准确。
Aim: To establish a determination method for the content of the effective flavonoids in leaf of Crataegus pinnatifida Bge. Method: The HPLC system column, mobile phase of CH 3CN 3%CH 3COOH (11∶89) mixture, with detection wave at 270 nm, was used to determine the content of vitexin in Leaf of Crataegus pinnatifida Bge. The mean recovery of 99.09%, RSD=3.76% for vitexin were obtained. Result: The method is sensitive and accurate with good reproducibility.
出处
《时珍国医国药》
CAS
CSCD
2000年第10期871-872,共2页
Lishizhen Medicine and Materia Medica Research
