摘要
目的通过观察咳喘宁对呼吸道合胞病毒(RSV)诱发哮喘模型大鼠CD4+、CD8+T细胞内信号转导子和转录激活子6(STAT6)表达的影响,阐明咳喘宁防治RSV诱发支气管哮喘的机理。方法分别从磷酸盐缓冲液(PBS)对照组、模型组大鼠外周血中提取单个核细胞,采用免疫磁珠法分离获得CD4+、CD8+T细胞并对其行纯度鉴定与活力检测。采用免疫荧光法,使用流式细胞仪测定T细胞内STAT6蛋白的表达水平,以中药咳喘宁进行干预,动态观察细胞培养过程中,咳喘宁对CD4+、CD8+T细胞中STAT6蛋白表达的调节作用。结果免疫磁珠法获得的CD4+、CD8+T细胞纯度与活力均支持实验的下行进展。与PBS对照组比较,模型组CD4+T细胞含量显著升高(P<0.01),CD8+T细胞含量升高不明显(P>0.05)。模型组大鼠CD4+T与CD8+T细胞内STAT6的表达增高,咳喘宁治疗组CD4+与CD8+T细胞内STAT6的表达水平比模型组显著下降,差异有统计学意义(P<0.01);与地塞米松、沙丁胺醇组比较,咳喘宁组CD4+、CD8+T细胞内STAT6的表达水平略高,但差异无统计学意义(P>0.05)。结论咳喘宁通过下调哮喘模型大鼠CD4+、CD8+T细胞内STAT6的表达,纠正哮喘中Th2细胞的过度增殖分化,影响Th2免疫应答优势,从而减少伏痰的产生,达到防治支气管哮喘的目的 。
Objective To observe the impacts of Kechuanning on expression of signal transducer and activator of transcription 6 （STAT 6） in CD4^＋, CD8^＋T lymphocytes of athma rats induced by virus, and analyze the mechanism of Kechuanning in treating asthma induced by RSV. Methods The PBMC was isolated from the peripheral blood, CD4^＋T, CD8^＋T lymphocyte was separated by immunomagnetic beads. The purity and activity of CD4＋T and CD8＋T lymphocytes were measured by using flow cytometry, trypan-blue dye exclusion test. The protein expression of STAT6 was detected by fluorescence-activated cell sorting. Results The descending progress of the trial were supported by the purity and activity of CD4^＋T, CD8^＋T lymphocyte. CD4＋T lymphocyte in the asthma model group were increased （P〈0.01）, however the increase of CD8＋T lymphocyte were not significant （P〉0.05）. The expression of the protein of STAT6 in the treated group with Kechuanning decoction were decreased than that in the model group （P〈0.01）. Compared with Dexamethasone and Salbutamol group, there were no significant differences （P〉0.05）. Conclusion Kechuanning decoction could decrease the protein of STAT6 in CD4^＋, CD8^＋T lymphocyte, thereby it could regulate the disorder of Th1/Th2, thus prevent and treat the asthmatic attack induced by virus.
出处
《中国中医药信息杂志》
CAS
CSCD
2013年第10期25-28,共4页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金青年基金(30901921)
