摘要
目的:研究山豆根(Sophora tonkinensis Gagnep)治疗哮喘的物质基础和作用机制。方法:首先对山豆根进行液相色谱–质谱联用分析,获得山豆根的化学成分,随后通过数据库TCMIP,获得山豆根成分所能预测的靶点。在CTD数据库中,查找出与哮喘相关靶点,通过韦恩图对山豆根化学成分的靶点与其哮喘相关靶点进行结合,得出二者相互交叉的部分,即为交集靶点。采用Cytoscape v3.8.0软件构建“成分–靶点–疾病”的网络关系,采用DAVID数据库对交集靶点进行KEGG通路分析。最后对交集靶点进一步分析,获得山豆根治疗哮喘的核心靶点。结果:获得127个交集靶点与12个山豆根治疗哮喘的活性成分,筛选出31条KEGG信号通路,进一步挑选出核心靶点7个。结论:本文对山豆根具有多成分、多靶点、多通路等特点的哮喘治疗方法进行了研究和分析,为今后哮喘的治疗提供了理论依据。
Objective: To study the material basis and mechanism of Sophora tonkinensis Gagnep in the treat-ment of asthma. Methods: Firstly, the chemical constituents of Sophora tonkinensis Gagnep were analyzed by liquid chromatography-mass spectrometry, and then the predicted targets of Sophora tonkinensis Gagnep were obtained by TCMIP database. In the CTD database, find out the asthma related targets, combine the targets of chemical components of Sophora tonkinensis Gagnep and its asthma related targets through Wayne diagram, and get the intersection part of the two. Those were the intersection targets. Cytoscape v3.8.0 software was used to construct the network relationship of “component-target-disease”, and DAVID database was used to analyze the KEGG pathway of the intersection targets. Finally, the intersection targets were further analyzed to obtain the core target of Sophora tonkinensis Gagnep in the treatment of asthma. Results: One hundred and twenty-seven intersecting targets and twelve active components of Sophora tonkinensis Gagnep were obtained, thirty-one KEGG signal pathways were screened, and seven core targets were further selected. Conclusion: This paper studied and analyzed the treatment of asthma with the characteris-tics of multi-components, multi-targets and multi-pathways, which provided a theoretical basis for the future treatment of asthma.
出处
《药物化学》
2022年第3期265-274,共10页
Hans Journal of Medicinal Chemistry
