摘要
在天然提取的甜菊醇糖苷中,甜菊糖苷含量最多,但具有苦涩味,影响甜菊糖的广泛应用。本实验以藤仓赤霉Gibberella fujikuroi为出发菌株,采用紫外(UV)和5-溴尿嘧啶(5-BU)复合诱变的方法,定向诱变将甜菊糖苷微生物转化为味道甘甜的莱鲍迪苷A的菌株,得到一株高产突变菌株MS05,其发酵后RA含量比出发菌株提高22.98%,比发酵前提高39.29%。
Stevioside is the most abundant in steviol glycosides, but tasted bitter. In this study, Gibberella fujikuroi was used as original strain. A mutant strain MS05 was obtained after treated with ultraviolet(UV) and 5-bromouracil (5-BU),which could transform stevioside into rebaudioside A,a kind of steviol glycosides tasted more sweet and less bitter. After fermentation RA content increased 22.98% than original strain,increased 39.29% than before fermentation.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第18期225-228,共4页
Science and Technology of Food Industry
基金
安徽省科技攻关(12010302065)
安徽高校省级自然科学研究项目(ki2013A024)
安徽大学研究生学术创新研究项目(yqh100123
10117700623)