摘要
多酚氧化酶是植物体内广泛存在的铜结合酶，催化一元酚或多元酚氧化生成“黑色素”，是引起果蔬等农产品褐变的主要原因。通过对已克隆的植物多酚氧化酶基因的分析，设计了一对简并引物，ＰＣＲ扩增了甘薯多酚氧化酶保守区的ｃＤＮＡ，序列分析结果表明，该ｃＤＮＡ含有５９１个核苷酸，编码１９７个氨基酸，与已发表的其它植物的多酚氧化酶保守区的ｃＤＮＡ具有很高的同源性。
Polyphenol oxidase(PPO) is a ubiquitous copper enzyme which catalyzes the oxi- dation of mono-and O-diphenols into O-diquinoies. PPO is believed to be primarily re- sponsible for deleterious browning of many fruits and vegetables. Conserved region of sweet potato PPO gene was cloned and sequenced through PCR using a pair of degenerte primer designed on the basis of analysis of cloned PPO gene sequences. Results show it consists of 591 nucleotides encoding 197 amino acids in all and has a very high homology to previously cloned PPO in other plants.
出处
《热带作物学报》
CSCD
2000年第2期65-68,共4页
Chinese Journal of Tropical Crops
关键词
甘薯
多酚氧化酶
克隆
序列分析
sweet potato polyphenol oxidase cloning sequence analysis
