摘要
目的探讨COX-2抑制剂对膀胱癌T24细胞株裸鼠成瘤性的影响。方法 BALB/c裸鼠27只分为3组,每只皮下接种膀胱癌T24细胞5×106活细胞数建立移植瘤动物模型。COX-2抑制剂药物干预组(吲哚美辛、塞来昔布)采用喂饲途径,吲哚美辛3mg/kg,塞来昔布10mg/kg;对照组给予生理盐水溶液药物的投给。30d后处死裸鼠,取瘤块称重,测量肿瘤体积,行免疫组化、半定量RT-PCR、Wester Blot检测移植瘤COX-2表达。结果对照组细胞接种裸鼠后第5天可见肿瘤长出,第7天各组均有肿瘤长出,第30天后用药组移植瘤生长较对照组明显减慢。免疫组化染色、RT-PCR、Western-blot结果均显示对照组COX-2表达明显,而药物干预组均少量表达。结论选择性与非选择性COX-2抑制剂在实验中表现出良好的抗肿瘤特性。
ObjectiveTo establish the nude mice xenograft models of bladder cancer cell T24 and to study the inhibitive effects of Cyclooxygenase2 （COX2）. Methods Each of 27 athymic BALB/c mice was injected with 5×106 T24 bladder cancer cells. The mice were then divided equally into three groups. One group received oral indomethacin 3 mg/kg per mouse daily, the other 10 mg/kg celecoxib per mouse daily, and the control group received normal saline. The nude mice were killed after 30 days, and the weight and volume of xenografts were recorded. Histological hematoxylinosin staining and immunocytochemical SABC technique were employed to explore the histopathological changes. The expression of COX2 in the xenografts was detected by RTPCR and Westernblot. Results On the 5th day after transplantation, xenografts were found in the control group. On the 7th day, xenografts were observed in the other two groups. The expression level of COX2 reduced and the weight and volume of the xenografts decreased more in the groups treated with indomethacin or celecoxib.Conclusions Selective and nonselective COX2 inhibitors can inhibit the growth of xenografts in nude mice.
出处
《现代泌尿外科杂志》
CAS
2013年第5期453-456,共4页
Journal of Modern Urology
基金
重庆市卫生局面上项目(No.06-2-061)
