摘要
目的 探讨钙通道阻滞剂维拉帕米对兔耳增生性瘢痕的作用。方法 建立兔耳外伤性皮肤早期瘢痕增生模型 ,将 2 4只兔随机平均分为 3组 ,每组 8只 ,各组瘢痕内分别注射维拉帕米 2 5 μl(0 .0 6 2 5mg) 瘢痕、曲安缩松 2 5 μl(1mg 瘢痕 )、生理盐水 2 5 μl(0 .2 2 5mg 瘢痕 ) ,1次 10d ,共 2次。观察瘢痕外形变化。治疗后 2 0d在瘢痕处取材 ,切片行HE及Masson染色。光镜下观察 ,计算瘢痕增生指数、切片内成纤维细胞数密度 ,计算机辅助图象分析测算切片内胶原纤维面密度。结果 与生理盐水组相比 ,维拉帕米组和曲安缩松组瘢痕均变软、变平 ,色泽变浅 ,瘢痕增生指数及胶原纤维面密度均降低 ,3组间成纤维细胞数密度无显著变化 ;维拉帕米组瘢痕增生指数高于曲安缩松组 ,胶原纤维面密度两组间无显著性差异。结论 外伤性兔耳早期增生性瘢痕局部注射维拉帕米可降低瘢痕内胶原含量 ,引起瘢痕萎缩。本实验条件下维拉帕米的促瘢痕萎缩作用弱于曲安缩松。
Objective To study the effect of intralesional verapamil on the hypertrophic scar in rabbit ear.Methods Established the acute model of dermal hypertrophic scar in the rabbit ear. 24 rabbits were randomly divided into 3 groups,each group contained 8 rabbits. Scars in the 3 groups were treated by intralesional verapamil hydrochloride 25μl(0.0625 mg/scar), triamcinolone acetonide 25 μl(1 mg/scar) and normal saline 25 μl(0.225 mg/scar), respectively, once 10 days, for 2 times. The treated scars were harvested on the 10th day after the second injection. HEstained and Massonstained sections were prepared. The Hypertrophic Index (HI) of the scar and the Numerical Density on Area (NA) of the fibroblast were calculated under microscope. The Area Density on Area (AA) of collagen was measured using a computerassisted morphometrical system. Results Compared with the salinetreated scars, the verapamiltreated and the steroidtreated scars appeared to be softer , flatter, and lighter in color; the HI of scar and the AA of collagen inthese two groups decreased significantly; there was no statistical difference in the NA of fibroblast among the three groups. The HI of scar in verapamiltreated group was bigger than that in steroidtreated group, however, the AA of collagen showed no statistical difference between these two groups.Conclusion Intralesional verapamil could induce scar degradation and reduction by interference of collagen metabolism in the acute model of dermal hypertrophic scar in the rabbit ear. In this study, the degradative effect of verapamil on scar was less than that of triamcinolone acetonide.
