摘要
目的 :在先前已建立了模拟人艾滋病感染者和患者的猴免疫缺陷病毒 (SIV)急性感染猴和中、晚期猴艾滋病 (SAIDS)模型的基础上 ,再摸索建立SIV慢性感染猴模型 ,以期为抗艾滋病药物的体内药效学评价研究提供更合适的动物模型。方法 :用SIVmac2 51感染恒河猴 17只 ,追踪观察其临床表现 ,体征变化 ,病程进展 ,血浆和全血病毒血症规律 ,淋巴细胞亚群CD4 的动态等。结果 :临床体征有特征性 ,病程较长 ,1年内死亡 2只 ( 11.8% )。血浆病毒血症水平在感染后 14d达高峰 ,感染 2 1d开始下降 ,60～ 360d检不出或低滴度。全血病毒血症水平在感染后 10d开始持续高滴度至 3个月 ,3个月后仍有半数以上持续高滴度 ,9个月后才下降。淋巴细胞亚群CD4 值感染后 14～ 30d略下降 ,后回升。结论 :SIV慢性感染猴模型临床表现、病毒学和免疫学的变化及病程进展类似人艾滋病感染者在进入艾滋病中、晚期前的慢性感染过程 。
Further exploration of the establishment of monkey model chronically infected with Simian acquired immunodeficiency virus (SIV) was conducted on the basis of previous studies of SIV monkey models and simian acquired immunodeficiency syndrome models. Seventeen Rhesus monkeys were infected with SIVmac?251. Items such as symptoms and signs, course of infection,plasma viremia and the whole blood, changes of CD 4 lymphocyte subgroup were observed periodically. The results showed that specific clinical manifestations occurred, disease course prolonged and 2 of 17 monkeys died of severe immunodeficiency. Plasma viremia reached the peak on 14th day after infection, began to decrease on 21st day, and remained low titer or underdetermined from 60th to 360th day. Whole blood viremia reached high titer from 10th to 90th day, and over half of the monkeys maintained high viremia level up to 9th month and then decreased afterwards. CD 4 lymphocyte level decreased from 14th to 30th day, and then increased. The above changes of clinical manifestations, virology and immunology are similar to cases with chronic infection of human immunodeficiency virus (HIV) in the early stage. And this will provide a suitable animal model for the research of anti-AIDS drugs.
出处
《广州中医药大学学报》
CAS
2000年第4期355-357,共3页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广东省科委攻关课题!(编号 :SKkF 9813)
关键词
免疫缺陷病毒
艾滋病
动物模型
SIMIAN ACQUIRED IMMUNODEFICIENCY SYNDROMES
DISEASE MODELS, ANIMAL
MACACA
