摘要
目的测试用抗-HuCD8+T细胞单克隆抗体方法去除中国恒河猴体内CD8+T细胞效果,为建立CD8+T细胞缺失恒河猴模型提供实验依据。方法抗-HuCD8+T抗体cM-T807在0、37、d注射2只中国恒河猴,不同的时间取外周血,腹股沟和腋下淋巴结。制备CD3/CD4/CD8标记的外周血和淋巴细胞悬液,流式法测定恒河猴外周血和淋巴结中CD8+T细胞的去除效果。结果注射了cM-T807的中国恒河猴,24 h后,外周血中的CD8+T细胞缺失99.9%,持续27 d;168 h后,淋巴结中CD8+T细胞缺失约90.0%,持续21 d。结论首次证实抗-HuCD8+T细胞抗体cM-T807能有效去除中国恒河猴体内CD8+T细胞,为建立CD8+T细胞缺失动物模型(CD8+T Cells del)奠定基础。
Objective To identify the effectiveness of McAb （cM-T807） anti-huCD8^＋T cell on eliminating the CD8^＋ lymphocytes from Chinese original rhesus monkeys. Methods The anti-CD8 ^＋ T cell McAb was injected into two rhesus monkeys at 0 d, 3 d and 7 d, respectively. Peripheral blood and lymph node samples were collected from the two monkeys at different time after injection. The CD3/CD4/CD8 from both peripheral blood lymphocytes （PBL）and lymph nodes （LN） cell suspension were differentiated by FACS and the effectiveness of elimination of CD8^＋ lymphocyte was detected. Results The results showed that 99.9% and 90.0% reduction of CD8~ cells in peripheral blood and lymph nodes from two Chinese-origin rhesus macaques were detected from 24 and 168 hours after injection, respectively, and the duration of low CD8^＋ levels maintained for 27 days in PBL and in LN for 21 days. Conclusions CD8^＋ T cells can be eliminated effectively from PBL and LN in Chinese original rhesus monkeys by injection of McAb cM-T807.
出处
《中国比较医学杂志》
CAS
2007年第6期321-324,共4页
Chinese Journal of Comparative Medicine
基金
国际合作项目
美国NIH资助(R01HL080211-01)
关键词
恒河猴
CD8^+T细胞缺失
模型
动物
Rrhesus macaques, Chinese origin
CD8^＋T cell elimination
Model, animal
