摘要
目的探讨点阵Er：YAG激光照射对紫外线所致豚鼠光老化皮肤胶原纤维和弹性纤维的影响。方法随机数字表法将60只豚鼠分为无处理组10只和模型组50只，中波紫外线（UVB）和长波紫外线（UVA）照射模型组豚鼠皮肤构建光老化模型，随机选择10只作为光老化模型组，余40只豚鼠分为2周、4周、8周、12周治疗组。点阵Er：YAG激光连续多脉冲照射治疗组豚鼠左侧背部（治疗侧）皮肤，HE染色、Masson染色、Weigertvan Gieson染色和碱水解-分光光度法比较照射后，治疗侧和对照侧皮肤组织学改变、胶原纤维、弹性纤维和羟脯氨酸含量。结果UVA和UVB的最小红斑量4224mJ／cm2和504mJ／cm2隔日照射豚鼠背部皮肤，累积照射UVA459．36J／cm2和UVB54．81J／cm2，成功获得光老化模型。HE染色显示，治疗组治疗侧皮肤真皮浅层激光刺激区新生的胶原纤维和弹性纤维增加、排列致密。12周组Masson染色胶原面积比值治疗侧（0．70±0．12）较对照侧（0．63±0．08）均有增加，差异有统计学意义（t=1．18，P〈0．05）；羟脯氨酸含量治疗侧[（4．73±0．39）mg／g]较对照侧[（3．66±0．85）mg／g]亦有增加，差异有统计学意义（t=3．40，P〈0．05）。结论点阵Er：YAG激光连续多脉冲照射可以刺激光老化豚鼠皮肤真皮浅层胶原纤维含量增加，纤细的弹性纤维新生。
Objective To evaluate the effect of fractional Er：YAG laser therapy on collagen and elastic fibers in photoaged skin of uhraviolet-irradiated Guinea pigs. Methods Sixty Guinea pigs were randomly divided into the non-intervention group （n = 10） and model group （n = 50） by a random number table. To establish a model of photoaging, the 50 Guinea pigs were irradiated by ultraviolet A （UVA） and ultraviolet B （UVB） on the back every other day for two months. Subsequently, the 50 mice were equally classified into five groups： photoaging group receiving no laser therapy, as well as 2-week, 4-week, 8-week and 12-week group receiving one, one, two and three sessions of successive muhi-pulsed fractional Er：YAG laser therapy, respectively, on the left side （treatment side） of the back, with the right side as the control side. The interval of laser therapy was four weeks. Skin biopsies were obtained from the right and left side of the back of Guinea pigs after the laser therapy, and subjected to haematoxylin and eosin （HE） staining, Masson staining and Weigert-van Gieson staining. The content of hydroxyproline was measured by using an alkaline-hydrolysis method. Results The minimal erythema dose for UVA and UVB was 4224 mJ/cm2 and 504 mJ/cm2 respectively on Guinea pig skin. The model for photoaging was successfully established in 50 Guinea pigs after two months of irradiation with the cumulative dose of UVA and UVB being 459.36 J/cm2 and 54.81 J/cm2 respectively. HE staining revealed an obvious increase in newly-growing collagen and elastic fibers, which were arranged densely, in the treatment side compared with the control side of the back of Guinea pigs after laser therapy. The area ratio of collagen fibers stained positive for Masson＇s trichrome and content of hydroxyproline were significantly higher in the treatment side than in the control side of the 12-week group （0.70 ± 0.12 vs. 0.63 ± 0.08, t = 1.18, P 〈 0.05; （4.73 ± 0.39） mg/g vs. （3.66 ± 0.85） mg/g, t = 3.40, P 〈 0
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2013年第9期648-651,共4页
Chinese Journal of Dermatology
关键词
皮肤衰老
激光疗法
小剂量
纤维胶原类
弹性组织
辐射损伤
实验性
Skin aging
Laser therapy, low-level
Fibrillar collagens
Elastic tissue
Radiation injuries, experimental
