摘要
对昆布多糖进行硫酸酯化修饰,考察修饰前后多糖结构及抗肿瘤活性的变化。采用氯磺酸-吡啶法进行多糖硫酸酯化修饰,考察了昆布多糖及其硫酸酯的红外光谱、核磁光谱特征,扫描电镜观察了表面形态,采用MTT比色法进行抗肿瘤活性评价。结果表明,昆布多糖及其硫酸酯都具有典型的多糖红外吸收,昆布多糖硫酸酯具有硫酸基的特征吸收峰;昆布多糖及其硫酸酯均是以β-(1→3)糖苷键为主链的多糖,昆布多糖硫酸酯的硫酸基取代位置在C2-OH与C6-OH。昆布多糖及其硫酸酯表面立体形态差异显著,昆布多糖表面呈云雾状或海绵状,昆布多糖硫酸酯表面呈片状或块状。昆布多糖及其硫酸酯对人肠癌细胞LOVO生长都具有明显的抑制作用,并且昆布多糖硫酸酯的抗肿瘤作用强于昆布多糖。
This study was aimed to sulfate modify laminarin and to investigate the relationship of structure and anti-tumor activity. Chlorosulfonie aeid-pyridine method was applied for sulfated modification of laminarin. IR and NMR spectra of laminarin and laminarin sulphate （LAMS） were investigated, arid the surface structure of laminarin and LAMS were ob- served with scanning electron microscope. The anti-tumor activities were evaluated by MTY method. The results showed that both laminarin and LAM$ showed characteristic absorption peaks of polysaccharide in IR spectra, and LAMS had characteristic absorption peaks of sulfate;the NMR spectra showed that laminarin and LAMS both had/3-（1--＊3） glyco- side bond as main chain, and the sulfate group in LAMS was attached to C2-OH and Ce-OH. Under scanning electron mi- croscope,there were obvious differences in surface structure between laminarin and LAMS. Laminarin was shown like cloud or sponginess, while LAMS was shown like flakiness or block. MTI＇ results showed both laminarin and LAMS had inhibitory effects on LOVO growth, and the anti-tumor activity of LAMS was higher. In conclusion, sulfated modification changed chemical structure of laminarin and enhanced its anti-tumor activity markedly.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2013年第8期1041-1045,1088,共6页
Natural Product Research and Development
基金
黑龙江省青年科学基金项目(QC2011C100)
黑龙江省教育厅科学技术研究项目(12511136)
关键词
昆布多糖
硫酸酯
修饰
抗肿瘤
laminarin
sulfated polysaccharides
modification
anti-tumor activity
