摘要
目的探讨影响新发结核病患者结核菌检出率的因素,提高结核病的早期诊断率。方法收集河南省胸科医院2010年3月至2012年3月收治的新发结核病患者150例和健康志愿者25例,将上述人群分为四组:A组,痰涂片阳性肺结核;B组,痰涂片阴性肺结核;C组,肺外结核;D组,健康志愿者。对每个纳入的研究对象取3份标本分别进行抗酸杆菌涂片检测、改良罗氏(L-J)固体和MGIT960液体培养,得到常规留痰标本共309份,雾化引痰标本216份,共收集痰标本525份。结果 A组,雾化引痰的涂片阳性率、L-J培养检出率、MGIT960培养检出率均高于常规取痰(P<0.05);B、C组,雾化引痰的L-J和MGIT960培养阳性率均高于常规取痰(P<0.05)。L-J培养阳性率12.8%,MGIT960培养阳性率26.5%,两者差异有统计学意义(P<0.05)。结论雾化引痰比常规取痰的涂片、培养检查阳性率高,初诊疑似结核患者应送检雾化引痰标本,进行结核菌涂片和MGIT960培养检测。涂片阴性肺结核和肺外结核患者也可能通过呼吸道低量排菌,这在结核病的防控中时应给予足够的重视。
Objective To study the impact of different methods for sputum specimen collection on tubercle bacilli positive rate of new tuberculosis patients. Methods Sputum specimens were collected from 150 new tuberculosis patients and 25 healthy volunteers in Henan Chest Hospital from March 2010 to March 2012. People enrolled in the study were divided into 4 groups：group A,sputum smear-positive pulmonary tuberculosis; group B, sputum smear-negative pulmonary tuberculosis; group C, extrapulmonary tubercu- losis;group D, healthy volunteers. 3 specimens were got from each person,which were detected by acid-fast bacillus smear,L-J solid and MGIT960 liquid culture medium, respectively. 309 regular sputum specimens, 216 atomization-induced sputum specimens, and a total of 525 sputum specimens were collected. Results Compared between atomization-induced and regular sputum,in group A,the positive rates of sputum smear,L-J culture,and MGIT960 culture were higher than the regular sputum（P〈0. 05） ;in group B and C,the positive rates of MGIT960,L-J culture were higher than regular sputum（P〈0.05）. The positive rate of L-J culture was 12.8% ,MGIT960 was 26.5% ,the difference was statistically significant（P〈0.05）. Conclusion For patients newly diagnosed pa- tients with suspected tuberculosis,atomization-induced sputum samples should be used for TB smear and MGIT960 culture tests. Smear-negative pulmonary and extrapulmonary tuberculosis patients may also discharge bacteria of low level through respiratory tract, to which adequate attention should be given in the prevention and control of tuberculosis.
出处
《国际检验医学杂志》
CAS
2013年第15期1941-1942,共2页
International Journal of Laboratory Medicine
基金
中美结核病科研合作项目(10-I-N060)
关键词
结核
痰
培养
涂片
tuberculosis
sputum
cultivation
smear
