摘要
用共聚焦显微镜对不同原代培养细胞的p38丝裂原活化蛋白激酶(mitogen -activatedproteinkinase,MAPK)进行定位 ,以探讨 p38激活后入核在不同的细胞中是否具有普遍性。发现与单核细胞相似 ,未受刺激静止的心肌细胞、平滑肌细胞和内皮细胞的 p38荧光强度呈弥散性分布;脂多糖(lipopolysaccharide,LPS)刺激后 ,细胞核区荧光强度均明显增加 ,胞浆荧光强度均降低。RAW细胞对LPS诱导p38移位入核的敏感性较其它细胞高 ;在上述4种细胞LPS刺激引起的p38的移位入核速度也不相同。这表明 p38蛋白激酶移位入核依赖于其磷酸化激活 ,且在多种细胞具有普遍性;但在不同细胞LPS诱导 p38移位入核的速度和敏感性有一定差别。
To investigate if p38 translocation to nuclei is a universal event, the distribution of p38 mitogen-activated protein kinase(MAPK) in different primary cultured cells was detected by laser scanning confocal microscope. Similar to that in RAW cells, it was found that fluorescence-labeled p38 spread all over the cytosol and nucleiin the rest cardiomyocytes, smooth muscles cells and endothelial cells, while the fluorescent intensity in cytosol area decreased and that in nuclear area increased after the stimulation of LPS. The LPS-induced response of nuclear translocation of p38 in RAW cells was more sensitive than that in other cells checked here. The speed of nuclear translocation of p38 in these four kind cells stimulated with LPS was also different. The data herein indicated that the nuclear translocation of p38 is dependent on phosphorylated activation and this phenomenon is universal in many kind cells. However, the moving speed and sensitivity of p38 translocation in different cells induced by LPS is different.
出处
《生物物理学报》
CAS
CSCD
北大核心
2000年第3期481-488,共8页
Acta Biophysica Sinica
基金
国家杰出青年科学基金!(39925014)
国家自然科学基金!重点(39830400)
面上项目! (39800074
39870332)
军队杰出人才基金!(98J003
关键词
P38丝裂原活化蛋白激酶
细胞内定位
信号转导
p38 mitogen-activated protein kinase Intracellular localization Signal transduction
