摘要
目的：观察大鼠视神经夹伤后玻璃体腔内注射睫状神经营养因子（CNTF）和腺病毒介导脑源性神经营养因子（Ad-BDNF）对视神经损伤后视网膜神经节细胞（RGC）存活的影响。方法：制作大鼠视神经定量夹伤模型，玻璃体腔内注射CNTF和Ad-BDNF，经上丘荧光金（FG）逆行标记RGC，计数视网膜铺片上的RGC并行统计学分析。结果：正常SD大鼠视网膜上RGC密度为2155±265个／mm。（n=12），视神经夹伤后RGC在1～2wk内下降速率最快，到3，4wk时RGC细胞数量虽仍有减少但下降速度已经明显减慢。CNTF组在视神经夹伤后1wk时视网膜RGC数显著高于对照组，但2～4wk的结果和对照组比较差异不明显。Ad—BDNF组视神经夹伤后1～4wk视网膜RGC数均显著高于对照组。结论：CNTF治疗组玻璃体腔内一次性注射CNTF可以在损伤早期2wk内为损伤的RGC提供神经营养因子，减少RGC的早期死亡。Ad．BDNF治疗组的这种保护作用可以持续到损伤后4wk，能够为RGC提供长时间地营养支持，但这种作用比较局限，可能与单一营养因子作用有关。
AIM： To investigate the effect of intravitreal injection of CNTF and Ad-BDNF on survival of RGC after optic nerve injury.
METHODS： Optic nerve crush model in rats were established. CNTF and Ad-BDNF were intravitreal injected by retrograde marking the RGC with fluoro-gold （FG）. The retrograde labeled RGC were counted under fluorescent microscope.
RESULTS： The number of RGC counted by retrograde labeling of FG was about 2155 ± 265个/mm^2 （n = 12） after label. Number of RGC decreased quickly after crush 1-2 weeks. In CNTF group, the number of RGC was higher than that of control group （ P〈 0.05）. But the number of RGC 2-4 weeks after crush in CNTF group had no difference with that in control group. In Ad-BDNF group, the number of RGC 2-4 weeks after crush were significant difference compared with that of control group at every time point （P〈0.05）.
CONCLUSION ： Intravitreal injection of CNTF can protect the RGC in the early time. But these effects can not work for more than 2 weeks. Intravitreal injection of Ad-BDNF can protect the RGC in 4 weeks.
出处
《国际眼科杂志》
CAS
2008年第6期1130-1132,共3页
International Eye Science
基金
中国人民解放军兰州军区科研基金项目(No.LXH-2005008)~~
