摘要
目的:探讨EphrinA1-Fc对人肾透明细胞癌786-O细胞系促红细胞生成素产生肝细胞受体A2(EphA2)和细胞外调节蛋白激酶1/2(ERK1/2)磷酸化程度的影响。方法:应用可溶性配体EphrinA1-Fc干预人肾透明细胞癌786-O细胞系,采用Wstern blot方法分析不同时间点细胞内EphA2和ERK1/2的磷酸化程度。结果:EphrinA1-Fc干预5、10、30、60 min后,p-EphA2、p-ERK的相对表达量逐渐增加(F=9.392,P=0.025;F=4.428,P=0.041),p-EphA2、p-ERK在EphrinA1-Fc干预前均未见表达。结论:Eph rinA1-Fc抑制肿瘤转移复发的可能机制之一是其促使肾透明细胞癌786-O细胞EphA2磷酸化而导致其降解实现。
Objective： To detect the effect of EphrinA1-Fc on the phosphorylation of EphA2 and extracellular signal-regulated ki- nase （ERK） in 786-O renal carcinoma cells （RCCs）. Methods： The soluble ligand EphrinA1-Fc was used to inhibit the 786-O RCCs in vitro. Western blot analysis was used to examine the phosphorylation of EphA2 and ERK1/2 in the 786-0 RCCs at different time points. Results： After the intervention with EphrinA1-Fc for 5, 10, 30, and 60 min, the expression of p-EphA2 increased （F=9.392, P= 0.025） as well as that of p-ERK （F=4.428, P=0.041）. No p-EphA2 and p-ERK expression was observed in the pre-intervention group. Conclusion： One of the possible mechanisms of the inhibitory effect of EphrinA1-Fc on tumor metastasis and recurrence involves the phosphorylation of EphA2 by EphrinA1-Fc, leading to the degradation of EphA2.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2013年第16期956-959,共4页
Chinese Journal of Clinical Oncology
基金
河北省科技计划项目(编号:10276177)资助~~
