摘要
以中国野生葡萄14个种为材料,对控制花色苷合成的mybA转录因子进行克隆和序列分析,获得VvmybA1和VlmybA2两个转录因子的全长基因序列,共检测到121个SNP,表现出丰富的遗传多样性。3种中性检测方法比较序列变异模式,结果表明,中国野生种葡萄VvmybA1和VlmybA2基因没有偏离中性模型,反映出基因漂移和选择性中性突变之间的平衡。不同野生种材料的mybA基因结构存在很高的同源相似性。但是在启动子区、内含子区以及第3个外显子区存在不同程度碱基的缺失、插入和替换,而且野生种葡萄mybA基因存在一些特有序列或突变,这些突变可以作为分子标记区分不同的野生种材料。通过基因结构比对和系统进化树分析,可将野生种葡萄细分为5个类群。初步推测桦叶葡萄和变叶葡萄进化地位较为原始。
The transcription factor corresponding to the mybA that regulates anthocyanin biosynthesis was cloned and analyzed on a sample of 14 Chinese wild grapes species. Two complete mybA-related gene sequences, VvmybA1 and VlmybA2 were obtained, and a total of 121 SNPs were detected in the sequenced fragments which give a high lev- el of polymorphism. Three neutrality tests had been used to compare the patterns of sequence variations, the result in- dicates a non departure from neutrality expectations for gene VvmybA1 and VlmybA2 of Chinese wild grape which reflected equilibrium between genetic drift and selectively neutral mutation. The mybA gene acquired form different wild accessions represented a highly homology. Yet extensive base deletion, insertion and substitution existed in pro- motor region, intron region and the third coding exon, which exhibited rich genetic diversity between species of Chi- nese wild grapes. Furthermore, there were several unique bases or mutations in the mybA gene of wild grapes that might be selected to be the molecular marker to distinguish themselves from others well. Comparing and analysising the gene structure and phylogenetic tree of the gene sequence, the studied Chinese wild grape species were divided into five groups. Moreover, V. betulifolia and V. hancockii were initially predicted to be primitive in the evolution.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2013年第5期885-891,共7页
Journal of Plant Genetic Resources
基金
现代农业产业技术体系建设专项资金(CARS-30-yz-1)
农业部作物种质资源保护项目(NB2012-2130135-34)
