摘要
为进一步了解福建福安刺葡萄的遗传背景,寻找刺葡萄与欧亚种葡萄之间的遗传差异,本研究基于高通量测序技术展开对福安刺葡萄基因组的分析,共得到测序数据8.4Gb,鉴定出3 192 484处非冗余遗传差异。差异位点分布位置结果显示,30.34%的差异位于基因间区,3.67%的差异位于基因外显子区。通过对比葡萄参考基因组序列,共鉴定出刺葡萄基因组上存在1 863 237个同源SNP、1 244 590个异源SNP位点,表明本次测序所用的福安刺葡萄有可能是异源二倍体。
Genetic variants in Vitis davidiifrom Fuan District in Fujian were studied using the high-throughput genome sequencing method.The 8.4Gb of raw data were analyzed to result in the identification of 3 192 484 nonredundant genetic variants.Distribution of the variants showed 30.34% in the intergenic regionand 3.67% in the exon region.By comparing the genome sequences of the V.davidii under study with those of the entire grape references,1 863 237homo-type SNP and 1 244 590hetero-type SNP were found.It appeared that the V.davidii from Fuan were most likely to be allodiploidy.The study also indicated that using genetic sequencing to determine the genome-wide variants of V.davidii was a viable tool for better understanding of the genetic background of the plant in question.
出处
《福建农业学报》
CAS
北大核心
2017年第2期134-137,共4页
Fujian Journal of Agricultural Sciences
基金
福建省科技计划项目--省属公益类科研院所基本科研专项(2014R1014-9)
现代农业产业技术体系建设专项资金(CARS-30)
关键词
高通量测序
福安刺葡萄
基因组
high-throughput sequencing
Vitis davidii
genome
