摘要
设计合成了一种可逆的Hg2+荧光增强型分子探针3',6'-双(二乙氨基)-2-((2-羟基-4-甲氧基苯亚甲基)氨基)螺[异吲哚-1,9'-氧杂蒽]-3-硫酮(RBS),并利用红外光谱、元素分析、核磁等方法对探针结构进行表征。探针本身荧光很弱,与Hg2+结合后荧光显著增强,由此,建立了Hg2+测定的新方法。该方法对Hg2+测定的线性范围为5.00×10-9～1.10×10-6mol/L,检出限为2.82×10-9mol/L,具有操作简单、灵敏度高、选择性好、线性范围宽等特点。将该方法用于河水及土壤样品中Hg2+的加标回收实验,结果满意。
A novel fluorescence enhancing probe for Hg^2＋, 3＇, 6＇-bis（diethylamino）-2-（（2-hy droxy-4-methoxybenzylidene） amino） spiro [ isoindoline-1, 9＇-xanthene ] -3-thione （ RBS）, was de signed and synthesized. Its structure was characterized with IR, elemental analysis and ^1H NMR spectra. The fluorescence intensity of probe solution is very weak, but after combination with Hg^2＋ , its fluorescence intensity increased obviously. Thus, a new method for the determination of Hg2 ＋ was established. It exhibits a dynamic response concentration range for Hg^2＋ from 5.00 x 10-9 mol/L to 1.10 x 10-6 mol/L with a detection limit of 2. 82 x 10 -9 mol/L. The method showed advantages of simplicity, high sensitivity, excellent selectivity and wide linear range, and was applied in the de- termination of Hg^2＋ in real samole with satisfactorv res.h.
出处
《分析测试学报》
CAS
CSCD
北大核心
2013年第8期947-952,共6页
Journal of Instrumental Analysis
基金
国家自然科学基金资助项目(21175083)
关键词
荧光光谱法
荧光探针
汞离子(Ⅱ)
罗丹明B类衍生物
fluorescent spectrometry
fluorescent probe
mercury ion （ 11 ）
rhodamine B derivatives
