摘要
目的 :观察抑制或拮抗内源性碱性成纤维细胞生长因子 (b FGF)对肠缺血再灌注所致肠、肝、肾功能的影响。方法 :96只大鼠分成 b FGF抗体预处理、丝氨酸苏氨酸蛋白激酶抑制剂 H7预处理以及生理盐水对照3组 ,肠系膜上动脉根部用微血管夹夹闭 45分钟之后放松血管夹形成再灌注。另 6只作为正常对照 (假手术组 )。分别于伤后即刻、2、6、2 4和 48小时将动物活杀 ,取血检测肝、肾功能以及二胺氧化酶 (DAO)变化 ;取肠、肝、肾组织进行形态学观察。结果 :与假手术组相比 ,3组动物血浆肝、肾以及肠道损伤指标均明显升高 ,其中伤后 2和 6小时 b FGF抗体组、H7组以及生理盐水对照组动物血浆丙氨酸转氨酶 (AL T)分别比对照组平均增加2 .5至 3.5倍 (P<0 .0 5或 P<0 .0 1) ;血浆 DAO变化及病理学结果均提示伤后 2和 6小时 3组动物肠屏障功能显著破坏。结论 :抑制或拮抗内源性 b FGF活性将进一步加重缺血再灌注所致肠、肝、肾损伤 ,其主要作用环节是影响了 b FGF与受体的结合以及随之发生的信号传导。
Objective:To explore the effects of inhibition or antiendogenous basic fibroblast growth factor (bFGF)on functional changes of intestine,liver and kidneys in rats after gut ischemiareperfusion insult.Methods:Ninetysix Wistar rats were divided into four groups,namely bFGF antibody pretreated group(A),H7 pretreated group(B),normal saline control (C) and shamoperated controls (D).In group A and B,all rats were pretreated with bFGF antibody or H7 before 45minute superior mesenteric artery (SMA) occlusion.Blood samples were collected at 2,6,24,and 48 hours after reperfusion,and organ functional changes were examined.Tissue samples from intestine,liver and kidneys were also taken for histological analysis.Results:SMA occlusion induced the tissue damage of intestine,liver and kidneys.In rats pretreated with bFGF antibody,H7 and normal saline,the functional parameters of liver,kidneys and intestine were markedly changed compared with those in shamoperated rats.Serum alanine aminotransferase and blood urea nitrogen levels were more than 2 5 to 3 0fold when compared with shamoperated controls.The changes of gut histochemical examination and serum diamine oxidase indicated the intestinal barrier was injured.Conclusions:Inhibition or antiendogenous bFGF may further cause the functional change and histochemical damage in intestine,liver and kidneys.
出处
《中国危重病急救医学》
CSCD
2000年第8期465-468,共4页
Chinese Critical Care Medicine
基金
国家杰出青年科学基金资助项目!( 3 95 2 5 0 2 4)
全军"九五"重点课题基金资助项目!( 98Z0 90 )
关键词
损伤
缺血-再灌注
肠道
抗体
H7
BFGF
ischemiareperfusion injury
gut
liver
kidney
basic fibroblast growth factor
antibody
H7
