摘要
AIM： To observe the therapeutic effect of early administration of exogenous Basic fibroblast growth factor （bFGF） on acute edematous pancreatitis （AEP） in rats. METHODS： Thirty male Sprague-Dawley rats were randomly divided into three （n = 10）： normal control group （group Ⅰ）, AEP group （group Ⅱ） and AEP with bFGF treatment group （group Ⅲ）. AEP was induced by subcutaneous injection of cerulein （5.5 μg/kg and 7.5 μg/kg） at 1 h interval into rats of groups Ⅱ and Ⅲ. Three hours after induction of AEP, 100 μg/kg bFGF was administrated intraperitoneally for 1h to group Ⅲ rats. For test of DNA synthesis in acinar cells, 5-bromo-2＇-deoxyuridine （BrdU） labeling solution was intraperitoneally injected into the rats of groups Ⅱ and Ⅲ 24 h after bFGF treatment. The changes in serum amylase, lipase, pancreatic tissue wet/dry ratio were detected. RESULTS： In bFGF treatment group, there was a significant decrease in the volume of serum amylase, lipase and the pancreatic wet/dry weight ratio（1383.0 ± 94.6 U/L, 194.0 ± 43.6 U/L, 4.32 ± 0.32） compared to AEP group （3464 ± 223,7 U/L, 456 ±68,7 U/L, 6.89 ± 0,47） （P 〈 0.01）, and no significant difference was found between bFGF treatment and control group （1289 ± 94.0 U/L, 171 ± 23.4 U/L, 4.12 ± 0.26, P 〉 0.05）. The inflammatory changes such as interstitial edema, polymorphonuclear neutrophUs （PMNs） and vacuolization were significantly ameliorated compared to AEP group （P 〈 0.01）. A small number of BrdU-labeled nuclei were observed in acinar cells of AEP rats （1.8 ± 0.3 nuclei/microscopic field, n = 10） while diffuse BrdU-labeled nuclei were found in bFGF-treated rats （18.9 ± 1.4 nuclei/microscopic field, n = 10） （P 〈 0.01）. Immunohistochemical study showed increased DNA synthesis in pancreatic acinar cells. CONCLUSION： Early administration of exogenous bFGF has significant therapeutic effect on cerulein-induced acute edematous pancreatitis in rats. Its mechanism is re
瞄准：在老鼠在尖锐水肿胰腺炎(AEP ) 上观察外长的基本成纤维细胞生长因素(bFGF ) 的早管理的治疗学的效果。方法：三十只男 Sprague-Dawley 老鼠随机被划分成三(n = 10 ) ：正常控制组(组我) ， AEP 组(组 II ) 和有 bFGF 处理的 AEP 组织(组 III ) 。AEP 被 cerulein (5.5 microg/kg 和 7.5 microg/kg ) 的皮下注射在 1 h 间隔导致进组 II 和 III 的老鼠。在 AEP 的正式就职以后的三个小时， 100 microg/kg bFGF intraperitoneally 被管理让 1 h 组织 III 老鼠。为在腺泡房间的 DNA 合成的测试，把答案标记的 5-bromo-2'-deoxyuridine (BrdU ) intraperitoneally 在 bFGF 处理以后被注入组 II 和 III 24 h 的老鼠。在浆液淀粉酶的变化，脂肪分解酵素，胰腺的织物湿 / 干燥的比率被检测。结果：在 bFGF 处理组，在浆液淀粉酶，脂肪分解酵素和胰腺的湿/干燥的重量比率的体积有重要减少( 1383.0+/-94.6 U/L ， 194.0+/-43.6 U/L ， 4.32+/-0.32 )与 AEP 组相比( 3464+/-223.7 U/L ， 456+/-68.7 U/L ， 6.89+/-0.47 )( P < 0.01 )，并且没有有效差量在 bFGF 处理和控制组之间被发现( 1289+/-94.0 U/L ， 171+/-23.4 U/L ， 4.12+/-0.26 ， P > 0.05 )。象空隙的浮肿， polymorphonuclear neutrophils (PMN ) 和空泡形成那样的煽动性的变化显著地与 AEP 组相比被改善(P < 0.01 ) 。标记 BrdU 的原子核的一个小数字在 AEP 老鼠的腺泡房间被观察(1.8+/-0.3 nuclei/microscopic 地， n = 10 ) 当弥漫的标记 BrdU 的原子核在对待 bFGF 的老鼠被发现时(18.9+/-1.4 nuclei/microscopic 地， n = 10 )(P < 0.01 ) 。Immunohistochemical 学习在胰腺的腺泡房间显示出增加的 DNA 合成。结论：外长的 bFGF 的早管理在老鼠在导致 cerulein 的尖锐水肿胰腺炎上有重要治疗学的效果。它的机制与发炎的改善和胰腺的新生的容易有关。
