摘要
旨在研究动物抗血清在抗幽门螺杆菌感染方面的免疫交叉保护作用。以幽门螺杆菌标准株基因组为模板,PCR特异性扩增尿素酶A亚基基因,构建表达载体pBVIL1-ureA并转化大肠杆菌,诱导转化菌表达,用纯化的表达蛋白免疫家兔得到家兔抗血清,用抗血清和病人血清进行ELISA试验。结果显示,原核表达的抗原蛋白IL1-UreA既能与兔抗血清反应也能与幽门螺杆菌感染的病人血清反应,且两种血清与抗原蛋白存在竞争性结合反应。动物抗血清具有免疫保护作用,可以利用动物特异性抗体进行幽门螺杆菌感染的免疫预防和免疫治疗。
In this paper,immune cross protection from ureA antigen between rabbit antiserum and patient serum,infected by H.pylori,was tested.The ureA gene from H.pylori 26695 strain was amplified with PCR technology and cloned into expressive vector pBVIL1 and the recombinant pBVIL1-ureA was transformed into DH5α strain.The ureA antigen purified was used to immune rabbit three times and antiserum was acquired.The antiserum titre and immune cross protection were tested with ELISA.The results showed that the antigen expressed by prokaryotic system could specifically react on both patient serum and rabbit antiserum and possess a competitive immune cross reaction.As a result,it was concluded that animal antibodies against H.pylori may be applied in immune precaution and therapy of infections diseases from H.pylori.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第8期135-138,共4页
Biotechnology Bulletin
关键词
幽门螺杆菌
尿素酶A亚基
抗血清
免疫保护
Helicobacter pylori Urease subunit A Antiserum Immune protection
