摘要
有效抗原及表位的预测和筛选是疫苗研究的基础,在对鸡新城疫病毒HN蛋白抗原表位预测的基础上,对多表位抗原进行表达与免疫原性测定.根据生物信息学表位预测方法获得的家禽新城疫病毒抗原表位,利用PCR技术合成基因,构建p BVIL1-HN重组载体,转化大肠杆菌HB101,进行基因工程表达;经纯化蛋白后免疫小鼠,抗体滴度用酶联免疫吸附方法测定,确定抗原的免疫原活性.结果表明,多表位抗原基因经测序结果正确,融合基因在大肠杆菌得到高效表达,电泳纯融合多表位抗原经三次免疫得到抗血清,抗体滴度为1∶8 000.鸡新城疫病毒HN蛋白多表位抗原得到高效表达,且具有良好的免疫原性.
On the basis of epitope prediction, multi-epitope antigen of hemagglutinin-neuraminidase（HN） of Newcastle disease virus（NDV） was expected to be constructed and expressed and its immunogenicity was detected using ELISA. The gene of predicted HN multi-epitope antigen of NDV was synthesized by PCR technique and subcloned into pBVIL1 vector. The antigen protein was expressed in E. coli strain HB101 and mice were immunized three times with purified antigen. Then antiserum titer was detected with ELISA. HN epitope antigen was expressed correctly and the antiserum immunogenicity against HN antigen was ,1 ： 8 000 titer. HN multiple epitope antigen of NDV was expressed high effectively in prokaryotie cells and showed better immunoge- nicity in mice.
出处
《山东理工大学学报（自然科学版）》
CAS
2008年第5期15-18,共4页
Journal of Shandong University of Technology:Natural Science Edition
关键词
新城疫病
HN抗原
多表位抗原
免疫原性
newcastle disease virus
hemagglutinin-neuraminidase
epitope
immunogenicity
