摘要
目的:探讨中医干预对艾滋病免疫重建不全患者TCRVβ基因CDR3区克隆片断的影响。方法:以15例HIV抗体阴性健康献血员做对照,采集37例艾滋病免疫重建不全患者治疗前后的外周血PBMC,采用人类TCRVβ基因CDR3多样性定量检测试剂盒检测,基因扫描作图并计算Vβ家族中每个家族不同大小CDR3区片段的分布。结果:对比正常人,患者一些Vβ内出现了明显的CDR3区的单寡克隆扩增情况,治疗后各家族单寡克隆情况有不同程度的改善和恢复。其中9,11,21,224个家族的D值2组治疗后均比治疗前下降,治疗组下降幅度较对照组在21,22 2个家族更为显著(P<0.05)。治疗组显著降低第18家族D值,而对照组则显著增加(P<0.05)。结论:中药复方对于TCRVβ各家族单寡克隆情况有不同程度的改善和恢复,提示中医药可能促进T细胞部分受体基因重排,丰富受体库,帮助机体免疫细胞有效识别病毒,减少T细胞凋亡。
Objective： To discuss the drug intervention in diversity changes of TCRVβ gene in AIDS patients with incomplete immune reconstitution. Method： PBMCs were isolated from 37 cases of AIDS patients failure to immune reconstitution before and after treatment with immune 2 and 15 cases of HIV negative healthy donors. The human gene TCRVβ CDR3 diversity quantitative detection reagent box were used, and mapped the distribution of gene scanning and calculated different CDR3 fragme of each Vβ family size. Result： Compared with the normal group, there appeared some single or oligoclonal amplification of Vβ CDR3 region in the patients, which were improved or recovered after treatment. Among them, D value of four families （9, 11, 21, 22 ） decreased after treatment in both groups. The decrease in family 21 and 22 was significant （P〈0.05） in treatment group compared with the control group. And family 18 was decreased in treatment group and increased significantly in control group （P〈0.05）. Conclusion： Study of the mechanism showed oligoclonal of TCRVβ family can get recovery in some degrees after treated by Immune 2 plus HAART, suggesting that the medicine may promote T-cell receptor gene rearrangement, helping immune cells to effectively identify the virus to reduce T-cell apoptosis.keywords：AIDS
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第15期2424-2428,共5页
China Journal of Chinese Materia Medica
基金
国家"艾滋病和病毒性肝炎等重大传染病防治"科技重大专项(2008ZX10005-004)
