摘要
目的探讨甲胎蛋白异质体3(AFP-L3)、高尔基体蛋白73(GP73)、热休克蛋白27(HSP27)在原发性肝癌患者中的诊断价值。方法收集98例患者血清,其中原发性肝癌36例,肝转移癌14例,胃癌20例,健康对照28例,采用电化学发光法检测AFP,采用酶联免疫吸附试剂盒检测各组血清中AFP-L3、HSP27、GP73的表达情况。结果 AFP、AFP-L3、GP73、HSP27在原发性肝癌组的表达情况明显高于肝转移癌组、胃癌组及健康对照组,明显差异有统计学意义(P<0.01)。通过受试者工作特征曲线(ROC)评价作图,AFP、AFP-L3、GP73及HSP27曲线下面积分别为:0.969、0.977、0.978、0.985。AFP-L3、GP73及HSP27与AFP的相关性为0.896、0.853、0.838(P<0.05);AFP、AFP-L3、GP73及HSP27与乙肝表面抗原的相关性为0.076、0.119、0.176、0.176(P>0.05)。结论 AFP-L3、HSP27、GP73均可以作为原发性肝癌的肿瘤标记物,与AFP相比,具有更高的诊断价值,它们与表面抗原的表达情况无关。
Aim Discuss the values of Alpha-fetoprotein-L3,Golgi protein 73 and Heat shock protein27 in the diagnosis of primary hepatic carcinoma. Methods AFP was measured by electro-chemiluminescence immunoassay.Enzyme-linked immunosorbent assay （ELISA） was used to detect the serum level of AFP-L3, HSP27 ,GP73 in 36 cases of primary hepatic carcinoma,14 cases of metastatic liver cancer,20 cases of gastric cancer and 28 cases of healthy control. Results Serum AFP,AFP-L3,HSP27,GP73 were significantly higher in patients with primary hepatic carcinoma compared with other groups（P〈0.05）.Areas under the ROC curves of AFP, AFP-L3,HSP27,GP73wereO.969,0.977,0.978,0.985.The correlation between AFP and AFP-L3,HSP27,GP73 were0.896,0.853,0.838 （P〈0.05）.the correlation between HBV SAg and AFP, AFP-L3,HSP27,GP73 were 0.119,0.176,0.176.（P〉0.05）. Conclusion AFP-L3, HSP27,GP73 could be used as biomarkers in the diagnosis of primary hepatic carcinoma,compared with AFP, they have better values,it has no correlation with HBV SAg.
出处
《中外医疗》
2013年第13期26-28,共3页
China & Foreign Medical Treatment
