摘要
针对热杀菌肉制品中因微生物残留而引起的腐败问题,选择从腐败的玉米热狗肠中分离出并由Biolog微生物自动分析系统鉴定的地衣芽孢杆菌为代表菌株,结合地衣芽孢杆菌的特异性引物,用聚合酶链式反应(PCR)方法构建致腐微生物溯源技术。结果表明,该方法可在腐败样品中准确、灵敏、快速地检出地衣芽孢杆菌,引物的特异性为100%,方法的检测限为0.104CFU/g。
Thermally sterilized meat products may still become spoiled due to the presence of residual microorganisms.In the present study,a PCR-based technique was developed for tracing spoilage microorganisms in meat products.For this,a set of specific primers was designed for Bacillus licheniormis as a representative strain of meat spoilage microorganisms,isolated from spoiled corn hot dog sausage and identified with the Biolog microbial identification system.The PCR-based method allowed accurate,sensitive and rapid detection of Bacillus licheniormis in spoiled samples with a 100% primer specificity and a detection limit of 0.104 CFU/g.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第14期178-181,共4页
Food Science
基金
"十二五"国家科技支撑计划项目(2012BAD28B00)
