摘要
目的采用2种前处理方法检测茶叶中有机磷类农药,以此对比不同方法间的提取差异。方法采用QuEChERS与固相萃取2种不同的前处理方法对茶叶样品进行前处理,用毛细管气相色谱法脉冲火焰光度检测器对处理后样品中的有机磷类农药残留量进行测定,并对不同前处理方法的回收率进行比较。结果 2种方法平均回收率经统计学软件SPSS13.0统计分析,敌敌畏:χ2=10.89,P<0.01;乐果:χ2=9.36,P<0.05;甲基对硫磷:χ2=8.83,P<0.05;马拉硫磷:χ2=9.23,P<0.05;对硫磷:χ2=6.75,P<0.05。结论 2种方法加标回收率差异具有统计学意义,提取差异较大。QuEChERS法检测有机磷残留的前处理方法虽然简单、快捷,但是回收率低于国家标准,不适合检测基体较复杂的茶叶样品。
[ Objective ] To compare the extraction difference between two pretreatment methods in determination of organophosphorus residues in tea. [ Methods ] The tea samples were pretreated by two methods, which included QuEChERS and solid phase extrac- tion. After pretreatment, the levels of organophosphorus residues in samples were detected by capillary gas chromatography with pulsed flame photometric detector, and the recovery rate of different pretreatment methods were compared. [ Results] By SPSS 13.0 analysis, the difference in average recovery rate between two methods were listed as followed： Dichlorvos X^2 = 10.89, P 〈0. 01; Rogor X^2 = 9.36, P 〈 0.05 ; Methyl parathion X^2 = 8.83, P 〈 0.05 ; Malathion X^2= 9.23, P 〈 0.05 ; Parathion X^2 = 6.75, P 〈0.05. [ Conclusion] There is significant difference in standard addition recovery rate between two methods, and the extraction difference is significant. QuEChERS is simple and fast, but the recovery rate is lower than the national standard, so it is not suit- able for the detection of complex samples of tea.
出处
《职业与健康》
CAS
2013年第15期1894-1896,共3页
Occupation and Health
关键词
气相色谱
茶叶
有机磷
前处理
Gas chromatography
Tea
Organic phosphorus
Pretreatment
