摘要
目的研究犀角地黄汤对D-氨基半乳糖胺(D-gala ctosamine,D-GalN)/脂多糖(lipopolysaccharide,LPS)小鼠急性肝衰竭模型血浆中细胞因子的影响,以探讨犀角地黄汤对该模型保护作用的部分机制。方法采用腹腔注射D-GalN/LPS建立小鼠急性肝衰竭模型。犀角地黄组应用犀角地黄汤灌胃,模型对照组用等体积双蒸水灌胃。应用流式微球技术(cytometric bead array,CBA)检测3 h正常空白组、模型对照组、犀角地黄组小鼠血浆中细胞因子水平。结果犀角地黄汤组小鼠肿瘤坏死因子-α(tumor necrosis factorα,TNFα)为(709.44±336.12)pg/ml,与模型对照组相比,对急性肝衰竭模型小鼠3 h TNF-α无降低作用;IL-10为(994.67±589.63)pg/ml,与模型对照组相比,对3 h IL-10有升高作用(P<0.05)。结论犀角地黄汤对急性肝衰竭模型有保护作用,其作用机制不在于降低TNF-α分泌水平,而是提高IL-10以抑制过强的炎性反应。
Objective To study the effect of Xijiao Dihuang decoction on the plasma cytokine levels of D - galactosamine （D -GalN）/lipoplysaccharides （LPS） induced acute liver failure mouse model, and to elucidate the potential mechanism responsible for the protective effect on this mouse model by Xijiao Dihuang decoction. Methods The mouse model of acute liver failure was developed by intraperitoneally injecting D - GalN/LPS. The Xijiao Dihuang group and the control group were intragastically administered with Xijiao Dihuang decoction and double distilled water, respectively. The cytokine level 3 h after treatment in each group was assayed by cytometric bead assay. Results The level of Tumor necrosis factor - α （ TNF - α） in the Xijiao Dihuang group was （ 709.44± 336.12） pg/ml. Compared with the control group, no significant TNF -α reduction was observed in the mouse model of acute liver failure 3 h after Xijiao Dihuang decoction treatment. In contrast, the level of IL -10 in Xijiao dihuang group was （994.67± 589.63） pg/ml 3 h after treatment that was significantly elevated as compared with the control group （P 〈 0.05）. Conclusions Xijiao Dihuang decoction generates protective effect on the mouse model of acute liver failure. The underlying mechanism may be up - regulation of IL -10 production rather than reduction of TNF -α secretion, thereby inhibiting the enhanced proinflammatory responses.
出处
《武警医学》
CAS
2013年第7期605-607,共3页
Medical Journal of the Chinese People's Armed Police Force
