摘要
目的 观察CD3单抗、CD2 8单抗、PHA和低剂量辐射对淋巴细胞增殖的影响 ,探讨影响T淋巴细胞信号转导的因素。方法 分别以CD3单抗、CD2 8单抗、PHA和低剂量辐射作为作用因素 ,用3H TdR掺入法观察在这些因素作用下 ,淋巴细胞增殖反应情况。结果 CD3单抗和CD2 8单抗联合刺激、PHA和低剂量辐射单独或联合处理 ,均可使淋巴细胞明显增殖。但CD3单抗和CD2 8单抗单独刺激 ,淋巴细胞增殖不明显。结论 CD3单抗和CD2 8单抗联合作用可使淋巴细胞有效增殖 ,PHA和低剂量辐射具有相似作用 。
Objective Effects of CD3 McAb,CD28 McAb,PHA and lowdose γray irradiation on T lymphocytes were investigated to explore factors of influencing T cell signals transduction.Method Using CD3 McAb and CD28 McAb minicking as the first and the second signals,and using PHA and low dose γrays irradiation as sitimulatory factors in T cell activation,the influences of these factors and the two signals on human lymphocyte proliferation response were studied with 3H thymidin incorporation.Results Lymphocyte proliferation response occurred when the two signals were treated costimulaneously or within certain intervals (within 40h).PHA and 10cGy γrays irradiation can also activate lymphocytes to proliferate.However,each of the two signals alone did not activate lymphocytes to proliferatc.Conclusion CD3 McAb,CD28 McAb,PHA and lowdose γrays irradiation could stimulate T lymphocyte proliferation,which is an important aspect in cellular immune regulation.
出处
《苏州医学院学报》
2000年第7期614-616,共3页
Acta Academiae Medicinae Suzhou
