摘要
为了解粘附分子（Ａｄｈｅｓｉｏｎｍｏｌｅｃｕｌｅｓ）在Ｔ细胞激活中的作用及其机制，作者采用ＴＣＲ－ＣＤ３复合物、ＬＦＡ－１、ＬＦＡ－２、ＣＤ４４和ＣＤ４５特异性单克隆抗体刺激，ＦｃγＲＩＩ阳性或阴性肿瘤细胞刺激，以及刺激阻断的方法，以ＩＦＮ－γ定量检测作为Ｔ细胞激活的标志，研究了ＴＣＲ－ＣＤ３复合物交联（Ｃｒｏｓｓ－ｌｉｎｋｉｎｇ），交联的不同形式，ＬＦＡ－１、ＬＦＡ－２、ＣＤ４４和ＣＤ４５分子对Ｔ细胞激活的影响及其机制。结果提示：ＴＣＲ－ＣＤ３复合物的持久性交联是Ｔ细胞体外激活的必要条件，上述４种粘附分子是Ｔ细胞激活的共刺激分子，其作用机制不仅是细胞间的粘附，而且还作为各自独立的刺激分子影响Ｔ细胞的信号传递。
For investigating the roles of adhesion molecules in T cell were activation and the mechanism, T cells were stimulated with monocolonal antibodies directed at TCR-CD3 complex, LFA-1, LFA-2, CD44 and CD45 molecules, and Fc_γ RⅡ positive or negative tumor cells. In addition, T Cells or K562 cells were incubated with the indicated McAbs for inhibition assays. Amounts of IFN-γ produced by T cells under the stimulation and inhibition was quantitated as the marker of T cell activation. Our data suggested that T cell activation is dependent on the cross-linking of the TCR-CD3 complex. The binding of four adhesion molecules to their counter receptors resulted not only in cell-cell adhesion, but also in signal transduction by functioning as independent costimulators.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1995年第3期218-222,共5页
Academic Journal of Second Military Medical University
关键词
T淋巴细胞
细胞粘附分子
T-lymphocytes
cell adhesion molecule
cross-linking
cytokine
