摘要
探讨HLA -DR .DQ基因多态性与骨关节结核的遗传关联性 ,比较骨关节结核与肺结核之间易感基因的差异。方法 采用聚合酶链反应 -序列特异性引物 (PCR -SSP)方法 ,对 86例骨关节结核 ,88例正常人及 34例肺结核的HLA -DR .DQ等位基因进行分析。结果 骨关节结核组与正常人比较 ,骨关节结核组DRB1 0 9、DQB1 0 30 1基因频率增高 (38.99%比 8.2 4 % PC <0 .0 0 0 1 RR =8.92 ;18.2 7%比 2 .6 6 % PC <0 .0 5 RR =2 .2 1) ,DRB1 13.2基因频率显著低于对照组 (4 .76 %比 2 0 .50 % PC <0 .0 0 1 RR =0 .18)。骨关节结核组与肺结核比较 ,DRB1 0 9、DQB1 0 30 1基因频率增高 (38.99%比 9.2 % PC <0 .0 0 0 1;18.15%比 1.85% PC <0 .0 0 1) ,而DRB1 15基因频率低于肺结核组 (17.17%比 36 .3% PC <0 .0 1)。结论 HLA -DRB1 0 9、DQB1 0 30 1可能是我国骨关节结核的易感基因 ,DRB1 13.2为保护基因。
Objective To study the association with HLA-DR.DQ genes in these people with Tuberculosis of the bone and joint.susceptible gene was compared Tuberculosis of the bone and joint with pulmonary Tuberculosis.Methods The alleles at the HLA-DR.DQ typing were analyzed by polymerase chain reaction with sequence-specific primers(PCR-SSP)in 86 patients with tuberculosis of the bone and joint,34 patients with pulmonary tuberculosis and 88 healthy controls.Results Compared tuberculosis of the bone and joint with normal people,A significant increased frequency of DRB1*09 and DQB1*0301 was found in tuberculosis of the bone and joint(38.99% vs 8.24% PC<0.0001 RR=8.92;18.27% vs 2.66% PC<0.05 RR=2.21).The freuqency of DRB1*13.2 in tuberculosis of the bone and joint was significantly decreased(4.76% vs 20.50% PC<0.01 RR=0.18).Compared tuberculosis of the bone and joint with pulmonary tuberculosis,A significant increased frequency of DRB1*09 and DQB1*0301 was found in tuberculosis of the bone and joint(38.99% vs 9.2% PC<0.0001;18.15% vs 1.85% PC<0.01).The frequency of DRB1*15 in tuberculosis of the bone and joint was significantly decreased(17.17% vs 36.3% PC<0.01).Conclusion The results showed that HLA-DRB1*09 and DQB1*0301 were probably susceptible gene in tuberculosis of the bone and joint,HLA-DRB1*13.2 may be a protective gene.It was different for susceptible gene between tuberculosis of the bone and joint and pulmonary tuberculosis.
出处
《中国防痨杂志》
CAS
2000年第3期130-133,共4页
Chinese Journal of Antituberculosis
